P-glycoprotein expression is decreased in placenta accreta and placenta previa disorders.

Autor:	Bloise E; Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil - ebloise@icb.ufmg.br.; Department of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada - ebloise@icb.ufmg.br.; Department of Biomedical, Experimental and Clinical Sciences "Mario Serio, " University of Florence, Florence, Italy - ebloise@icb.ufmg.br., Seidita I; Department of Biomedical, Experimental and Clinical Sciences "Mario Serio, " University of Florence, Florence, Italy., Nardi E; Section of Pathology, Department of Health Sciences, University of Florence, Florence, Italy., Abati I; Department of Biomedical, Experimental and Clinical Sciences "Mario Serio, " University of Florence, Florence, Italy., Borba Vieira DE Andrade C; Department of Histology and Embryology, State University of Rio de Janeiro, Rio de Janeiro, Brazil., Castiglione F; Section of Pathology, Department of Health Sciences, University of Florence, Florence, Italy., Mecacci F; Department of Biomedical, Experimental and Clinical Sciences "Mario Serio, " University of Florence, Florence, Italy., Donati C; Department of Biomedical, Experimental and Clinical Sciences "Mario Serio, " University of Florence, Florence, Italy., Petraglia F; Department of Biomedical, Experimental and Clinical Sciences "Mario Serio, " University of Florence, Florence, Italy.
Jazyk:	angličtina
Zdroj:	Minerva obstetrics and gynecology [Minerva Obstet Gynecol] 2024 Sep 25. Date of Electronic Publication: 2024 Sep 25.
DOI:	10.23736/S2724-606X.24.05488-5
Abstrakt:	Background: P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP) are multidrug resistance (MDR) transporters that function as placental gatekeepers, lowering the fetal levels of diverse xenobiotics and toxins that may be circulating in the maternal blood throughout pregnancy. Placenta accreta spectrum (PAS) and the placenta previa (PP) disorders are obstetric pathologies encompassed by an abnormal invasion of chorionic villous tissue in the uterine wall or at the endocervical os, respectively. Given the fact that MDR transporters are involved in placentation and are highly responsive to inflammation, we hypothesized that immunostaining of P-gp and BCRP would be altered in PAS and in PP specimens. Methods: A total of 32 placental histological specimens, sorted in control (N.=8; physiological pregnancies), PAS (N.=14), and PP (N.=10), were subjected to immunohistochemistry for P-gp and BCRP transporters. Semi-quantitative scoring of the resulting immunostained area and intensity was undertaken. Results: Decreased P-gp staining intensity in the syncytiotrophoblast of the PAS compared to the control group (P<0.05) and in the PP compared to the PAS group was detected (P<0.05). Fetal blood vessel P-gp immunostaining was decreased in PAS and PP groups (P<0.001). Conclusions: We conclude that PAS and PP histological specimens exhibit decreased immunostaning of the drug transporter P-gp, and that fetuses born from these pregnancies may be exposed to greater levels of drugs and toxins present at the maternal circulation. Futures studies should attempt to investigate the mechanisms underlying P-gp down-regulation in these obstetric pathologies.
Databáze:	MEDLINE
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