Sub-acute bisphenol A exposure induces proteomic alterations and impairs male reproductive health in mice.

Autor: Yadav SK; ICMR-National Institute of Occupational Health (NIOH), Ahmedabad, India., Kumar A; International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, India., Yadav BG; Institute of Plant and Microbial Biology, Agricultural Technology Building, Taipei, Taiwan., Bijalwan V; ICMR-National Institute of Occupational Health (NIOH), Ahmedabad, India., Yadav S; ICMR-National Institute for Implementation Research on Non-Communicable Disease (NIIRNCD), Jodhpur, India., Patil GP; ICMR-National Institute of Occupational Health (NIOH), Ahmedabad, India., Sarkar K; ICMR-National Institute of Occupational Health (NIOH), Ahmedabad, India.; ICMR-National Institute of Cholera & Enteric Diseases (NICED), Kolkata, India., Palkhade R; ICMR-National Institute of Occupational Health (NIOH), Ahmedabad, India., Das S; ICMR-National Institute of Occupational Health (NIOH), Ahmedabad, India.; ICMR-National Institute of Cholera & Enteric Diseases (NICED), Kolkata, India., Singh DP; ICMR-National Institute of Occupational Health (NIOH), Ahmedabad, India.
Jazyk: angličtina
Zdroj: Journal of biochemical and molecular toxicology [J Biochem Mol Toxicol] 2024 Oct; Vol. 38 (10), pp. e23862.
DOI: 10.1002/jbt.23862
Abstrakt: Bisphenol A (BPA) is one of the most prevalent endocrine disrupting chemicals (EDCs) and there is widespread concern about the adverse effects of EDCs on human health. However, the exact mechanism of these toxicities has still not been fully deciphered. Additionally, studies have reported the toxicological effects at far low doses to the generally considered no-observed-adverse-effect level (NOAEL) dose. The present study investigates the effects of a sub-acute (28 days) exposure to BPA (10, 50 and 100 mg/kg/day) in adult male mice on various hormones levels, sperm motility, sperm count, functional integrity of sperm plasma membrane, testicular histological changes, oxidative stress markers and DNA damage. The key proteome signatures were quantified by LC-MS/MS analysis using Orbitrap Fusion Lumos Tribrid Mass Spectrometer equipped with nano-LC Easy-nLC 1200. Data suggest that the BPA exposure in all doses (below/above NOAEL dose) have greatly impacted the hormone levels, sperm parameters (sperm count, motility and membrane integrity) and testicular histology. Mass spectrometry-based proteomics data suggested for 1352 differentially expressed proteins (DEPs; 368 upregulated, 984 downregulated) affecting biological process, cellular component, and molecular functions. Specifically searched male reproductive function related proteins suggested a complex network where 46 potential proteins regulating spermatogenesis, sperm structure, activity and membrane integrity while tackling oxidative stress responses were downregulated. These potential biomarkers could shed some more light on our current understanding of the reproductive toxicological effects of BPA and may lead to exploration of novel interventions strategies against these targets for male infertility.
(© 2024 Wiley Periodicals LLC.)
Databáze: MEDLINE
Externí odkaz: