Characterization of Amniotic Fluid Ureaplasma Species from Pregnancies Complicated by Preterm Prelabor Rupture of Membranes.

Autor: Libra A; Biomedical Research Center, University Hospital Hradec Kralove, Sokolska 581, Hradec Kralove, 500 05, Czech Republic.; Generi Biotech, Hradec Kralove, Czech Republic., Bolehovska R; Institute of Clinical Microbiology, University Hospital, Hradec Kralove, Czech Republic.; Institute of Clinical Microbiology, Charles University, Medical faculty in Hradec Kralove, Hradec Kralove, Czech Republic., Kukla R; Institute of Clinical Microbiology, University Hospital, Hradec Kralove, Czech Republic.; Institute of Clinical Microbiology, Charles University, Medical faculty in Hradec Kralove, Hradec Kralove, Czech Republic., Musilova I; Biomedical Research Center, University Hospital Hradec Kralove, Sokolska 581, Hradec Kralove, 500 05, Czech Republic.; Institute of Clinical Microbiology, Charles University, Medical faculty in Hradec Kralove, Hradec Kralove, Czech Republic.; Department of Obstetrics and Gynecology, Hospital Most, Krajska Zdravotni a.s, Most, Czech Republic., Menon R; Division of Basic Science & Translational Research, Department of Obstetrics and Gynecology, Medical Branch, The University of Texas, Galveston, TX, USA., Jacobsson B; Department of Obstetrics and Gynecology, Institute of Clinical Science, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.; Region Västra Götaland, Department of Obstetrics and Gynecology, Sahlgrenska University Hospital, Gothenburg, Sweden.; Department of Genetics and Bioinformatics, Domain of Health Data and Digitalisation, Institute of Public Health, Oslo, Norway., Kacerovsky M; Biomedical Research Center, University Hospital Hradec Kralove, Sokolska 581, Hradec Kralove, 500 05, Czech Republic. kacermar@fnhk.cz.; Institute of Clinical Microbiology, Charles University, Medical faculty in Hradec Kralove, Hradec Kralove, Czech Republic. kacermar@fnhk.cz.; Department of Obstetrics and Gynecology, Hospital Most, Krajska Zdravotni a.s, Most, Czech Republic. kacermar@fnhk.cz.
Jazyk: angličtina
Zdroj: Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2024 Nov; Vol. 31 (11), pp. 3440-3451. Date of Electronic Publication: 2024 Sep 25.
DOI: 10.1007/s43032-024-01697-3
Abstrakt: The main aim of this study was to determine expanded sequence types (eSTs) of Ureaplasma species (U. spp.). DNA isolated from the amniotic fluid of pregnancies complicated by preterm prelabor rupture of membranes (PPROM) using an expanded multilocus sequence typing scheme. Additionally, the study sought to examine whether phylogenetic subgroups of U. spp. DNA differ with respect to maternal demographic and clinical parameters and selected aspects of short-term neonatal morbidity. This retrospective cohort study was focused on singleton pregnancies complicated by PPROM occurring between the gestational ages of 24+0 and 36+6 weeks, where amniocentesis was conducted to assess the intra-amniotic environment and the presence of U. spp. DNA in the amniotic fluid samples was confirmed. The stored aliquots of U. spp. DNA were used to assess differences in nucleotide sequences in six U. spp. genes (ftsH, rpL22, valS, thrS,ureG, and mba-np1) using the eMLST scheme. The expanded multilocus sequence typing scheme was performed in 73 samples of U. spp. DNA isolated from pregnancies complicated by PPROM. In total, 33 different U. spp. DNA eSTs were revealed, 21 (#20, 233-244, 248-251, 253, 255, 259, and 262) of which were novel. The most frequently identified eST was #41, identified in 18% (13/73) of the aliquots. Based on their genetic relationships, the U. spp. DNA was divided into two clusters and four subgroups [cluster I (U. parvum): A, 43% (n = 31); B, 15% (n = 11); and C, 26% (n = 19); cluster II (U. urealyticum): 1; 16% (n = 12)]. Cluster II had a higher rate of polymicrobial findings than cluster I (58% vs 16%; p = 0.005), while subgroup A had the highest rate of concomitant Mycoplasma hominis in the amniotic fluid samples (66%; p = 0.04). In conclusion, Ureaplasma spp. DNA obtained from PPROM consisted of 33 different eSTs of U. spp. DNA. No differences in maternal and neonatal characteristics were found among the phylogenetical subgroups of U. spp. DNA, except for a higher rate of polymicrobial amniotic fluid findings in those with U. urealyticumand the concomitant presence of M. hominis in the amniotic fluid in those with the presence of U. parvum.
(© 2024. The Author(s), under exclusive licence to Society for Reproductive Investigation.)
Databáze: MEDLINE
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