Macrophage uptake rate of Sonazoid in breast lymphosonography is highly conserved in healthy controls.

Autor: Tichauer KM; Biomedical Engineering, Illinois Institute of Technology, Chicago, IL 60616, United States of America., Machado P; Department of Radiology, Thomas Jefferson University, Philadelphia, PA, United States of America., Liu JB; Department of Radiology, Thomas Jefferson University, Philadelphia, PA, United States of America., Sarathchandra ASC; Biomedical Engineering, Illinois Institute of Technology, Chicago, IL 60616, United States of America., Stanczak M; Department of Radiology, Thomas Jefferson University, Philadelphia, PA, United States of America., Kraft WK; Department of Pharmacology, Physiology and Cancer Biology, Thomas Jefferson University, Philadelphia, PA, United States of America., Forsberg F; Department of Radiology, Thomas Jefferson University, Philadelphia, PA, United States of America.
Jazyk: angličtina
Zdroj: Physics in medicine and biology [Phys Med Biol] 2024 Oct 04; Vol. 69 (20). Date of Electronic Publication: 2024 Oct 04.
DOI: 10.1088/1361-6560/ad7f1c
Abstrakt: Subcutaneous microbubble administration in connection with contrast enhanced ultrasound (CEUS) imaging is showing promise as a noninvasive and sensitive way to detect tumor draining sentinel lymph nodes (SLNs) in patients with breast cancer. Moreover, there is potential to harness the results from these approaches to directly estimate cancer burden, since some microbubble formulas, such as the Sonazoid used in this study, are rapidly phagocytosed by macrophages, and the macrophage concentration in a lymph node is inversely related to the cancer burden. This work presents a mathematical model that can approximate a rate constant governing macrophage uptake of Sonazoid, k i , given dynamic CEUS Sonazoid imaging data. Twelve healthy women were injected with 1.0 ml of Sonazoid in an upper-outer quadrant of one of their breasts and SLNs were imaged in each patient immediately after injection, and then at 0.25, 0.5, 1, 2, 4, 6, and 24 h after injection. The mathematical model developed was fit to the dynamic CEUS data from each subject resulting in a mean ± sd of 0.006 ± 0.005 h -1 and 0.4 ± 0.1 h -1 for relative lymphatic flow ( EF l ) and k i , respectively. Furthermore, the roughly 25% sd of the k i measurement was similar to the sd that would be expected from realistic noise simulations for a stable 0.4 h -1 value of k i , suggesting that macrophage concentration is highly consistent among cancer-free SLNs. These results, along with the significantly smaller variance in k i measurement observed compared to relative lymphatic flow suggest that k i may be a more precise and promising approach of estimating macrophage abundance, and inversely cancer burden. Future studies comparing tumor-free to tumor-bearing nodes are planned to verify this hypothesis.
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Databáze: MEDLINE
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