Promising antibiofilm formation: Liquid phase pulsed laser ablation synthesis of Graphene Oxide@Platinum core-shell nanoparticles.

Autor: Hasoon BA; Department of Applied Sciences, University of Technology, Baghdad, Iraq., Hasan DMA; Department of Biomedical Engineering, Technology University, Baghdad, Iraq., Jawad KH; Department of Laser and Optoelectronics Engineering, University of Technology, Baghdad, Iraq., Shakaer SS; Department of Applied Sciences, University of Technology, Baghdad, Iraq., Sulaiman GM; Department of Applied Sciences, University of Technology, Baghdad, Iraq., Hussein NN; Department of Applied Sciences, University of Technology, Baghdad, Iraq., Mohammed HA; Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraydah, Qassim, Saudi Arabia., Abomughaid MM; Department of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Bisha, Bisha, Saudi Arabia., Ramesh T; Department of Physics, BVRIT Hyderabad College Engineering for Women, Hyderabad, India.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Sep 24; Vol. 19 (9), pp. e0310997. Date of Electronic Publication: 2024 Sep 24 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0310997
Abstrakt: The increasing prevalence of multi-drug resistance in pathogenic bacteria has rendered antibiotics ineffective, necessitating the exploration of alternative antibacterial approaches. Consequently, research efforts have shifted towards developing new antibiotics and improving the efficacy of existing ones. In the present study, novel core shell graphene oxide@platinum nanoparticles (GRO@Pt-NPs) and their unchanging form have been synthesized using the two-step pulsed laser ablation in liquid (PLAL) technique. The first step involved using the graphene target to create graphene nanoparticles (GRO-NPs), followed by the ablation of GRO-NPs inside platinum nanoparticles (Pt-NPs). To characterize the nanoparticles, various methods were employed, including UV-VIS, transmission electron microscopy (TEM), energy dispersive X-ray (EDX), mapping tests, and X-ray diffraction (XRD). The anti-bacterial and anti-biofilm properties of the nanoparticles were investigated. TEM data confirm the creation of GRO@Pt-NPs. The average particle size was 11 nm for GRO-NPs, 14 nm for Pt-NPs, and 26 nm for GRO@Pt-NPs. The results demonstrate that the created GRO@Pt-NPs have strong antibacterial properties. This pattern is mostly produced through the accumulation of GRO@Pt-NPs on the bacterial surface of Klebsiella pneumoniae (K. pneumoniae) and Enterococcus faecium (E. faecium). The inhibition zones against K. pneumoniae and E. faecium when GRO-NPs were used alone were found to be 11.80 mm and 11.50 mm, respectively. For Pt-NPs, the inhibition zones of E. faecium and K. pneumoniae were 20.50 mm and 16.50 mm, respectively. The utilization of GRO@Pt-NPs resulted in a significant increase in these values, with inhibitory rates of 25.50 mm for E. faecium and 20.45 mm for K. pneumoniae. The antibacterial results were more potent in the core-shell structure than the GRO-NPs alone or Pt-NPs alone. The current work uses, for the first time, a fast and effective technique to synthesize the GRO@Pt-NPs by PLAL method, and the preparation has high clinical potential for prospective use as an antibacterial agent.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Hasoon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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