Immunocompetent Brain Organoids with Microglia Allow Advanced Aging Research.

Autor:	Ferreira RO; Human Genome and Stem Cell Research Center, Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, Cidade Universitária, São Paulo, SP, Brazil. raianeferreira@ib.usp.br., Assoni AF; Human Genome and Stem Cell Research Center, Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, Cidade Universitária, São Paulo, SP, Brazil., da Silva MVR; Human Genome and Stem Cell Research Center, Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, Cidade Universitária, São Paulo, SP, Brazil., da Rocha LA; Human Genome and Stem Cell Research Center, Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, Cidade Universitária, São Paulo, SP, Brazil., de Castro MV; Human Genome and Stem Cell Research Center, Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, Cidade Universitária, São Paulo, SP, Brazil., Bertola D; Human Genome and Stem Cell Research Center, Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, Cidade Universitária, São Paulo, SP, Brazil., Zatz M; Human Genome and Stem Cell Research Center, Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, Cidade Universitária, São Paulo, SP, Brazil. mayazatz@usp.br.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024 Sep 25. Date of Electronic Publication: 2024 Sep 25.
DOI:	10.1007/7651_2024_565
Abstrakt:	Aging is a complex and multifactorial process that significantly affects brain function and health, since it is commonly associated with the emergence of neurodegenerative diseases. Recent advances in stem cell technology have facilitated the development of brain organoids, three-dimensional structures that mimic key aspects of brain architecture and functionality. By incorporating microglia, the resident monocyte-derived immune cells of the central nervous system, immunocompetent brain organoids can provide a more physiologically relevant model for studying brain aging. This chapter explores the methodology of immunocompetent brain organoids for advanced aging research, detailing protocols for their generation from a co-culture of neural stem cells and primitive macrophage progenitors. (© 2024. Springer Science+Business Media, LLC.)
Databáze:	MEDLINE
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