Core and accessory genomic traits of Vibrio cholerae O1 drive lineage transmission and disease severity.

Autor: Maciel-Guerra A; School of Veterinary Medicine and Science, University of Nottingham, College Road, Sutton Bonington, Loughborough, Leicestershire, LE12 5RD, UK., Babaarslan K; School of Veterinary Medicine and Science, University of Nottingham, College Road, Sutton Bonington, Loughborough, Leicestershire, LE12 5RD, UK., Baker M; School of Veterinary Medicine and Science, University of Nottingham, College Road, Sutton Bonington, Loughborough, Leicestershire, LE12 5RD, UK., Rahman A; NSU Genome Research Institute (NGRI), North South University, Baridhara, Bashundhara, Dhaka, 1229, Bangladesh., Hossain M; School of Veterinary Medicine and Science, University of Nottingham, College Road, Sutton Bonington, Loughborough, Leicestershire, LE12 5RD, UK.; NSU Genome Research Institute (NGRI), North South University, Baridhara, Bashundhara, Dhaka, 1229, Bangladesh., Sadique A; NSU Genome Research Institute (NGRI), North South University, Baridhara, Bashundhara, Dhaka, 1229, Bangladesh., Alam J; NSU Genome Research Institute (NGRI), North South University, Baridhara, Bashundhara, Dhaka, 1229, Bangladesh., Uzzaman S; Institute of Epidemiology, Disease Control and Research (IEDCR), 44, Shaheed Tajuddin Ahmed Sarani Mohakhali, Dhaka, 1212, Bangladesh., Ferdous Rahman Sarker M; Institute of Epidemiology, Disease Control and Research (IEDCR), 44, Shaheed Tajuddin Ahmed Sarani Mohakhali, Dhaka, 1212, Bangladesh., Sultana N; Institute of Epidemiology, Disease Control and Research (IEDCR), 44, Shaheed Tajuddin Ahmed Sarani Mohakhali, Dhaka, 1212, Bangladesh., Islam Khan A; International Centre for Diarrhoeal Disease Research, Bangladesh (icddr, b), 68, Shaheed Tajuddin Ahmed Sarani Mohakhali, Dhaka, 1212, Bangladesh., Ara Begum Y; International Centre for Diarrhoeal Disease Research, Bangladesh (icddr, b), 68, Shaheed Tajuddin Ahmed Sarani Mohakhali, Dhaka, 1212, Bangladesh., Hassan Afrad M; International Centre for Diarrhoeal Disease Research, Bangladesh (icddr, b), 68, Shaheed Tajuddin Ahmed Sarani Mohakhali, Dhaka, 1212, Bangladesh., Senin N; Department of Engineering, University of Perugia, 06125, Perugia, Italy., Hossain Habib Z; Institute of Epidemiology, Disease Control and Research (IEDCR), 44, Shaheed Tajuddin Ahmed Sarani Mohakhali, Dhaka, 1212, Bangladesh., Shirin T; Institute of Epidemiology, Disease Control and Research (IEDCR), 44, Shaheed Tajuddin Ahmed Sarani Mohakhali, Dhaka, 1212, Bangladesh., Qadri F; International Centre for Diarrhoeal Disease Research, Bangladesh (icddr, b), 68, Shaheed Tajuddin Ahmed Sarani Mohakhali, Dhaka, 1212, Bangladesh., Dottorini T; School of Veterinary Medicine and Science, University of Nottingham, College Road, Sutton Bonington, Loughborough, Leicestershire, LE12 5RD, UK. tania.dottorini@nottingham.ac.uk.; Centre for Smart Food Research, Nottingham Ningbo China Beacons of Excellence Research and Innovation Institute, University of Nottingham Ningbo China, Ningbo, 315100, P. R. China. tania.dottorini@nottingham.ac.uk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Sep 23; Vol. 15 (1), pp. 8231. Date of Electronic Publication: 2024 Sep 23.
DOI: 10.1038/s41467-024-52238-0
Abstrakt: In Bangladesh, Vibrio cholerae lineages are undergoing genomic evolution, with increased virulence and spreading ability. However, our understanding of the genomic determinants influencing lineage transmission and disease severity remains incomplete. Here, we developed a computational framework using machine-learning, genome scale metabolic modelling (GSSM) and 3D structural analysis, to identify V. cholerae genomic traits linked to lineage transmission and disease severity. We analysed in-patients isolates from six Bangladeshi regions (2015-2021), and uncovered accessory genes and core SNPs unique to the most recent dominant lineage, with virulence, motility and bacteriophage resistance functions. We also found a strong correlation between V. cholerae genomic traits and disease severity, with some traits overlapping those driving lineage transmission. GSMM and 3D structure analysis unveiled a complex interplay between transcription regulation, protein interaction and stability, and metabolic networks, associated to lifestyle adaptation, intestinal colonization, acid tolerance and symptom severity. Our findings support advancing therapeutics and targeted interventions to mitigate cholera spread.
(© 2024. The Author(s).)
Databáze: MEDLINE
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