Effects of mining activities and municipal wastewaters on element accumulation and integrated biomarker responses of the European chub (Squalius cephalus).

Autor: Nikolić D; University of Belgrade - Institute for Multidisciplinary Research, Department of Inland Waters Biology and Protection, Kneza Višeslava 1, 11030, Belgrade, Serbia. Electronic address: dusan@imsi.rs., Kostić J; University of Belgrade - Institute for Multidisciplinary Research, Department of Inland Waters Biology and Protection, Kneza Višeslava 1, 11030, Belgrade, Serbia., Đorđević Aleksić J; University of Belgrade - Institute for Multidisciplinary Research, Department of Inland Waters Biology and Protection, Kneza Višeslava 1, 11030, Belgrade, Serbia., Sunjog K; University of Belgrade - Institute for Multidisciplinary Research, Department of Inland Waters Biology and Protection, Kneza Višeslava 1, 11030, Belgrade, Serbia., Rašković B; University of Belgrade - Faculty of Agriculture, Institute of Animal Sciences, Nemanjina 6, Zemun, 11080, Belgrade, Serbia., Poleksić V; University of Belgrade - Faculty of Agriculture, Institute of Animal Sciences, Nemanjina 6, Zemun, 11080, Belgrade, Serbia., Pavlović S; University of Belgrade - Institute for biological research 'Siniša Stanković'-National Institute of the Republic of Serbia, Department of Physiology, Bulevar despota Stefana 142, 11060, Belgrade, Serbia., Borković-Mitić S; University of Belgrade - Institute for biological research 'Siniša Stanković'-National Institute of the Republic of Serbia, Department of Physiology, Bulevar despota Stefana 142, 11060, Belgrade, Serbia., Dimitrijević M; University of Belgrade - Institute for Multidisciplinary Research, Department of Life Sciences, Kneza Višeslava 1, 11030, Belgrade, Serbia., Stanković M; University of Belgrade - Institute for Multidisciplinary Research, Department of Life Sciences, Kneza Višeslava 1, 11030, Belgrade, Serbia., Radotić K; University of Belgrade - Institute for Multidisciplinary Research, Department of Life Sciences, Kneza Višeslava 1, 11030, Belgrade, Serbia.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2024 Oct; Vol. 365, pp. 143385. Date of Electronic Publication: 2024 Sep 21.
DOI: 10.1016/j.chemosphere.2024.143385
Abstrakt: This study aimed to determine concentrations of 29 elements in the gills and liver as well as biomarker response in gills, liver, and blood of European chub from Pek River (exposed to long-term mining activities), and to compare these findings with individuals from Ibar River (influenced by emission of treated municipal wastewater) and Kruščica reservoir (source of drinking water) using inductively-coupled plasma optical emission spectrometry (ICP-OES). The metal pollution index (MPI) was also calculated. Supporting analyses for the detection of the municipal wastewater presence at investigated localities included analyses of microbiological indicators (total coliforms and Escherichia coli) of faecal pollution. We have assessed biomarker responses from molecular to organism level using the condition index, comet assay, micronucleus test, oxidative stress parameters, histopathological alterations, and fluorescence spectroscopy parameters. Multibiomarker analysis was summarized by Integrated Biomarker Response v2 (IBRv2). Among these locations, Kruščica exhibited the lowest, whereas the Pek River displayed the highest values for both categories of indicator bacteria. Due to the porphyry copper ores mining, individuals from Pek River had several times higher Cu concentrations in both gills and liver compared to the other localities which was confirmed by biomarker responses and IBRv2 value. On the contrary, fish from Kruščica reservoir were the least affected by elemental pollution which is also confirmed by low MPI and IBRv2 values. Responses of biomarkers correspond to the elemental accumulation in the liver and gills of the Ibar River are positioned between the Pek River and Kruščica reservoir. Of all the biomarkers analyzed in this study, the condition index was the least sensitive. The results of this study showed that fluorescence spectroscopy may be a method for fast screening of structural changes in gills caused by the pollution burden.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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