Effects of intermittent exposure to hypobaric hypoxia and cold on skeletal muscle regeneration: Mitochondrial dynamics, protein oxidation and turnover.

Autor: Sánchez-Nuño S; Campus Docent Sant Joan de Déu, Universitat de Vic - Universitat Central de Catalunya (UVIC-UCC), C/ Sant Benito Menni, 18-20, 08830, Sant Boi de Llobregat, Spain., Santocildes G; Department of Cell Biology, Physiology and Immunology, Faculty of Biology, Universitat de Barcelona, Av. Diagonal 643, 08028, Barcelona, Spain. Electronic address: gsantocildes@ub.edu., Rebull J; Department of Cell Biology, Physiology and Immunology, Faculty of Biology, Universitat de Barcelona, Av. Diagonal 643, 08028, Barcelona, Spain., Bardallo RG; Department of Cell Biology, Physiology and Immunology, Faculty of Biology, Universitat de Barcelona, Av. Diagonal 643, 08028, Barcelona, Spain., Girabent-Farrés M; Campus Docent Sant Joan de Déu, Universitat de Vic - Universitat Central de Catalunya (UVIC-UCC), C/ Sant Benito Menni, 18-20, 08830, Sant Boi de Llobregat, Spain., Viscor G; Department of Cell Biology, Physiology and Immunology, Faculty of Biology, Universitat de Barcelona, Av. Diagonal 643, 08028, Barcelona, Spain., Carbonell T; Department of Cell Biology, Physiology and Immunology, Faculty of Biology, Universitat de Barcelona, Av. Diagonal 643, 08028, Barcelona, Spain., Torrella JR; Department of Cell Biology, Physiology and Immunology, Faculty of Biology, Universitat de Barcelona, Av. Diagonal 643, 08028, Barcelona, Spain.
Jazyk: angličtina
Zdroj: Free radical biology & medicine [Free Radic Biol Med] 2024 Nov 20; Vol. 225, pp. 286-295. Date of Electronic Publication: 2024 Sep 21.
DOI: 10.1016/j.freeradbiomed.2024.09.032
Abstrakt: Muscle injuries and the subsequent regeneration events compromise muscle homeostasis at morphological, functional and molecular levels. Among the molecular alterations, those derived from the mitochondrial function are especially relevant. We analysed the mitochondrial dynamics, the redox balance, the protein oxidation and the main protein repairing mechanisms after 9 days of injury in the rat gastrocnemius muscle. During the recovery rats were exposed to intermittent cold exposure (ICE), intermittent hypobaric hypoxia (IHH), and both simultaneous combined stimuli. Non-injured contralateral legs were also analysed to evaluate the specific effects of the three environmental exposures. Our results showed that ICE enhanced mitochondrial adaptation by improving the electron transport chain efficiency during muscle recovery, decreased the expression of regulatory subunit of proteasome and accumulated oxidized proteins. Exposure to IHH did not show mitochondrial compensation or increased protein turnover mechanisms; however, no accumulation of oxidized proteins was observed. Both ICE and IHH, when applied separately, elicited an increased expression of eNOS, which could have played an important role in accelerating muscle recovery. The combined effect of ICE and IHH led to a complex response that could potentially impede optimal mitochondrial function and enhanced the accumulation of protein oxidation. These findings underscore the nuanced role of environmental stressors in the muscle healing process and their implications for optimizing recovery strategies.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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