Electrospun polyvinyl alcohol nanofiber scaffolds incorporated strontium-substituted hydroxyapatite from sand lobster shells: synthesis, characterization, and in vitro biological properties.

Autor:	Diputra AH; Physics, Universitas Gadjah Mada, Sleman, Yogyakarta, Indonesia, Yogyakarta, Yogyakarta, 55281, INDONESIA., Dinatha IKH; Physics, Universitas Gadjah Mada, Sleman, Yogyakarta, Indonesia, Yogyakarta, Yogyakarta, 55281, INDONESIA., Cahyati N; Physics, Universitas Gadjah Mada, Sleman, Yogyakarta, Indonesia, Yogyakarta, Yogyakarta, 55281, INDONESIA., Fatriansyah JF; Universitas Indonesia, Depok, Depok, 16424, INDONESIA., Taufik M; Department of Chemistry, Faculty of Mathematic and Natural Science Universitas Sumatera Utara, Padang Bulan, Medan, 20155, INDONESIA., Hartatiek H; State University of Malang Department of Physics, Jl. Semarang 5, Malang, 65145, INDONESIA., Yusuf Y; Department of Physics, Universitas Gadjah Mada, Sekip Utara Bulaksumur, Yogyakarta, 55281, INDONESIA.
Jazyk:	angličtina
Zdroj:	Biomedical materials (Bristol, England) [Biomed Mater] 2024 Sep 23. Date of Electronic Publication: 2024 Sep 23.
DOI:	10.1088/1748-605X/ad7e92
Abstrakt:	The paper describes the synthesis of hydroxyapatite (HAp) and strontium-substituted hydroxyapatite (SrHAp) from sand lobster shells by a hydrothermal method. The HAp and SrHAp were incorporated into the polyvinyl alcohol (PVA) nanofiber scaffold through the eletrospinning method. The scaffolds were incorporated with 5wt% of hydroxyapatite (HAp), 5wt%, 10wt%, and 15% of SrHAp. The physicochemical, mechanical, and in vitro biological properties of the scaffold were evaluated. The incorporation of HAp or SrHAp was evidenced by the diffraction patterns and the phosphate functional groups related to HAp. The morphological results showed the decrement of fiber diameter in line with the increased SrHAp concentration. A tensile test was conducted to investigate the mechanical properties of the scaffolds, and the results showed that the scaffolds perform poorly at a higher SrHAp concentration because of exceeding agglomeration levels. The PVA/SrHAp15 performed the best antibacterial activity against E. coli and S. aureus with an inhibition zone of (15.2 ± 0.2) and (14.5 ± 0.8), respectively. The apatite formation was more abundant in PVA/SrHAp10 after immersion in a simulated body fluid (SBF). Cell viability results showed that the scaffold enabled the osteoblast cells to grow and proliferate. The biocompatibility of HAp and SrHAp resulted in the enhancement of cell adhesion. Based on all tests, the PVA/SrHAp 10 scaffold shows a strong candidate for further in vivo studies. (© 2024 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved.)
Databáze:	MEDLINE
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