Assessment of LAMPAuris for Rapid Detection of Candida auris in Clinical Specimens.

Autor: Yamamoto M; Department of Pediatrics, Teikyo University School of Medicine, Tokyo, Japan.; Institute of Medical Mycology, Teikyo University, Tokyo, Japan., Alshahni MM; Institute of Medical Mycology, Teikyo University, Tokyo, Japan., Komori A; Institute of Medical Mycology, Teikyo University, Tokyo, Japan., Mimaki M; Department of Pediatrics, Teikyo University School of Medicine, Tokyo, Japan., Makimura K; Institute of Medical Mycology, Teikyo University, Tokyo, Japan. makimura@med.teikyo-u.ac.jp.; Department of Medical Mycology, Graduate School of Medicine, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo, 173-8605, Japan. makimura@med.teikyo-u.ac.jp.
Jazyk: angličtina
Zdroj: Mycopathologia [Mycopathologia] 2024 Sep 23; Vol. 189 (5), pp. 87. Date of Electronic Publication: 2024 Sep 23.
DOI: 10.1007/s11046-024-00892-9
Abstrakt: Candida auris is a pathogenic yeast frequently exhibiting multidrug resistance and thus warrants special attention. The prompt detection and proper identification of this organism are needed to prevent its spread in healthcare facilities. The authors of this paper had previously developed LAMPAuris, a loop-mediated isothermal amplification assay, for the specific detection of C. auris. LAMPAuris is evaluated in this report for its ability to identify C. auris from five clades and to detect it from clinical specimens. A total of 103 skin swab samples were tested in comparison with a culture-based method and C. auris-specific SYBR green qPCR. The results show that the LAMPAuris assay had specificities ranging from 97 to 100% and sensitivities ranging from 66 to 86%. The lower sensitivity could be attributed to DNA degradation caused by the prolonged storage of the samples. In conclusion, LAMPAuris proved to be a rapid and reliable method for identifying C. auris and for detecting it in clinical specimens. Fresh specimens should ensure better yield and higher sensitivities.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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