| Autor: |
Almeida GHDR; Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Gibin MS; Department of Physics, State University of Maringá, Maringá, Brazil., Rinaldi JC; Department of Morphological Sciences, State University of Maringá, Maringá, Brazil., Gonzaga VHS; Department of Physics, State University of Maringá, Maringá, Brazil., Thom CR; Department of Morphological Sciences, State University of Maringá, Maringá, Brazil., Iglesia RP; The Ken & Ruth Davee Department of Neurology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA., da Silva RS; Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Fernandes IC; Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Bergamo RO; Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Lima LS; Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Lopomo B; Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Santos GVC; Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Nesiyama TNG; Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil., Sato F; Department of Physics, State University of Maringá, Maringá, Brazil., Baesso ML; Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Hernandes L; Department of Morphological Sciences, State University of Maringá, Maringá, Brazil., Meirelles FV; Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil., Carreira ACO; Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.; Center for Natural and Human Sciences, Federal University of ABC, Santo André, Brazil. |
| Abstrakt: |
Biomaterials derived from biological matrices have been widely investigated due to their great therapeutic potential in regenerative medicine, since they are able to induce cell proliferation, tissue remodeling, and angiogenesis in situ . In this context, highly vascularized and proliferative tissues, such as the uterine wall, present an interesting source to produce acellular matrices that can be used as bioactive materials to induce tissue regeneration. Therefore, this study aimed to establish an optimized protocol to generate decellularized uterine scaffolds (dUT), characterizing their structural, compositional, and biomechanical properties. In addition, in vitro performance and in vivo biocompatibility were also evaluated to verify their potential applications for tissue repair. Results showed that the protocol was efficient to promote cell removal, and dUT general structure and extracellular matrix composition remained preserved compared with native tissue. In addition, the scaffolds were cytocompatible, allowing cell growth and survival. In terms of biocompatibility, the matrices did not induce any signs of immune rejection in vivo in a model of subcutaneous implantation in immunocompetent rats, demonstrating an indication of tissue integration after 30 days of implantation. In summary, these findings suggest that dUT scaffolds could be explored as a biomaterial for regenerative purposes, which is beyond the studies in the reproductive field. |
