Deciphering nanoparticle protein coronas by capillary isoelectric focusing-mass spectrometry-based top-down proteomics.
| Autor: | Zhu G; Department of Chemistry, Michigan State University, 578 S Shaw Lane, East Lansing, Michigan 48824, USA. lsun@chemistry.msu.edu., Sadeghi SA; Department of Chemistry, Michigan State University, 578 S Shaw Lane, East Lansing, Michigan 48824, USA. lsun@chemistry.msu.edu., Mahmoudi M; Department of Radiology and Precision Health Program, Michigan State University, East Lansing, MI, USA., Sun L; Department of Chemistry, Michigan State University, 578 S Shaw Lane, East Lansing, Michigan 48824, USA. lsun@chemistry.msu.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2024 Oct 08; Vol. 60 (81), pp. 11528-11531. Date of Electronic Publication: 2024 Oct 08. |
| DOI: | 10.1039/d4cc02666g |
| Abstrakt: | The nanoparticle (NP) protein corona significantly influences the outcome of nanomedicine. We present the first example of top-down proteomics (TDP) measurement of the protein corona using capillary isoelectric focusing-mass spectrometry, identifying seventy proteoforms of 16 cancer-related genes. This technique has the potential to revolutionize our understanding of the protein corona and advance nanomedicine. |
| Databáze: | MEDLINE |
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