Human breast milk isolated lactic acid bacteria: antimicrobial and immunomodulatory activity on the Galleria mellonella burn wound model.

Autor:	Guarnieri A; Università degli Studi del Molise Department of Medicina e Scienze della Salute 'V. Tiberio', Campobasso, Italy., Venditti N; Università degli Studi del Molise Department of Medicina e Scienze della Salute 'V. Tiberio', Campobasso, Italy.; Unità Operativa (UO) Laboratorio Analisi, Responsible Research Hospital, Campobasso, Italy., Cutuli MA; Università degli Studi del Molise Department of Medicina e Scienze della Salute 'V. Tiberio', Campobasso, Italy., Brancazio N; Università degli Studi del Molise Department of Medicina e Scienze della Salute 'V. Tiberio', Campobasso, Italy., Salvatore G; Università degli Studi del Molise Department of Medicina e Scienze della Salute 'V. Tiberio', Campobasso, Italy., Magnifico I; Università degli Studi del Molise Department of Medicina e Scienze della Salute 'V. Tiberio', Campobasso, Italy., Pietrangelo L; Università degli Studi del Molise Department of Medicina e Scienze della Salute 'V. Tiberio', Campobasso, Italy., Falcone M; Università degli Studi del Molise Department of Medicina e Scienze della Salute 'V. Tiberio', Campobasso, Italy., Vergalito F; Università degli Studi del Molise Department of Agricultural, Environmental and Food Sciences, Campobasso, Italy., Nicolosi D; Università degli Studi di Catania Department of Drug and Health Sciences, Catania, Italy., Scarsella F; Università degli Studi del Molise Department of Medicina e Scienze della Salute 'V. Tiberio', Campobasso, Italy.; ASReM-Azienda Sanitaria Regionale del Molise, Campobasso, Italy., Davinelli S; Università degli Studi del Molise Department of Medicina e Scienze della Salute 'V. Tiberio', Campobasso, Italy., Scapagnini G; Università degli Studi del Molise Department of Medicina e Scienze della Salute 'V. Tiberio', Campobasso, Italy., Petronio Petronio G; Università degli Studi del Molise Department of Medicina e Scienze della Salute 'V. Tiberio', Campobasso, Italy., Di Marco R; Università degli Studi del Molise Department of Medicina e Scienze della Salute 'V. Tiberio', Campobasso, Italy.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2024 Sep 06; Vol. 14, pp. 1428525. Date of Electronic Publication: 2024 Sep 06 (Print Publication: 2024).
DOI:	10.3389/fcimb.2024.1428525
Abstrakt:	Introduction: Managing burn injuries is a challenge in healthcare. Due to the alarming increase in antibiotic resistance, new prophylactic and therapeutic strategies are being sought. This study aimed to evaluate the potential of live Lactic Acid Bacteria for managing burn infections, using Galleria mellonella larvae as an alternative preclinical animal model and comparing the outcomes with a common antibiotic. Methods: The antimicrobial activity of LAB isolated from human breast milk was assessed in vitro against Pseudomonas aeruginosa ATCC 27853. Additionally, the immunomodulatory effects of LAB were evaluated in vivo using the G. mellonella burn wound infection model. Results and Discussion: In vitro results demonstrated the antimicrobial activity of Lactic Acid Bacteria against P. aeruginosa . In vivo results show that their prophylactic treatment improves, statistically significant, larval survival and modulates the expression of immunity-related genes, Gallerimycin and Relish/NF-κB, strain-dependently. These findings lay the foundation and suggest a promising alternative for burn wound prevention and management, reducing the risk of antibiotic resistance, enhancing immune modulation, and validating the potential G. mellonella as a skin burn wound model. Competing Interests: Authors NV, MC, IM, LP, FV, SD, GSc, GP and RM were NATIVAL s.r.l. founders at the time of the study. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Guarnieri, Venditti, Cutuli, Brancazio, Salvatore, Magnifico, Pietrangelo, Falcone, Vergalito, Nicolosi, Scarsella, Davinelli, Scapagnini, Petronio Petronio and Di Marco.)
Databáze:	MEDLINE
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