Utilizing mechatronic agilent gas chromatography to validate therapeutic efficacy of Combretum paniculatum against oxidative stress and inflammation.

Autor:	Chukwuma IF; Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Enugu State, 410001, Nigeria.; Department of Genetics and Biotechnology, Faculty of Biological Sciences, University of Nigeria, Enugu State, 410001, Nigeria., Okafor KC; Department of Engineering, Faculty of Science and Engineering, Manchester Metropolitan University, M1 5GD, Manchester, UK.; Department of Electrical and Electronic Engineering Science, University of Johannesburg, Johannesburg, 2006, South Africa.; Department of Mechatronics Engineering, School of Electrical Systems Engineering and Technology (SESET), Federal University of Technology Owerri, 1526, Nigeria.; School of Engineering, University of Chichester, Bognor Regis, PO21 1HR, UK., Apeh VO; Department of Applied Sciences, Federal University of Allied Health Sciences, Enugu State, 01473, Nigeria., Nworah FN; Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Enugu State, 410001, Nigeria., Odo CP; Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Enugu State, 410001, Nigeria., Okafor IP; Cardiff Metropolitan University, Department of Applied Public Health, Cardiff, CF5 2YB, UK., Anoh K; School of Engineering, University of Chichester, Bognor Regis, PO21 1HR, UK., Anthony OC; Department of Mechatronics Engineering, School of Electrical Systems Engineering and Technology (SESET), Federal University of Technology Owerri, 1526, Nigeria.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2024 Aug 22; Vol. 10 (18), pp. e36586. Date of Electronic Publication: 2024 Aug 22 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2024.e36586
Abstrakt:	The quest for novel antioxidant and anti-inflammatory medications from medicinal plants is crucial since the plants contain bioactive compounds with a better efficacy and safety profile than orthodox therapy. This study harnesses the capabilities of mechatronics-driven Agilent Gas Chromatography, deploying in vitro, in vivo , and in silico models to unravel the antioxidant and anti-inflammatory attributes within Combretum paniculatum ethanol extract (CPEE). Employing gas chromatography-mass spectroscopy (GC-MS), our analysis efficiently segregates and evaluates volatile compound mixtures, a technique renowned for identifying organic compounds, as exemplified by its success in detecting fatty acids in food and resin acids in water. Using gas chromatography-mass spectrometry (GC-MS) and GC-FID analyses, this paper ascertains the comprehensive phytochemical composition of CPEE. Also, Molecular interactions of identified compounds with cyclooxygenase (COX-2) implicated in inflammatory urpsurge is verified. GC-MS and GC-FID analyses unveil 41 phytoconstituents within CPEE. Based on the in vitro research, CPEE demonstrated potential in inhibiting thiobarbituric acid-reactive substances, nitric oxide, and phospholipase lipase A2 with inhibition rates of 2.284, 6.547, and 66.8 μg/mL respectively. In vivo experiments confirm CPEE's efficacy in inhibiting granuloma tissue formation, lipid peroxidation, and neutrophil counts compared to untreated rats. Moreover, CPEE elicited a significant (P < 0.05) increase in the activities of SOD, CAT, and GSH concentrations while decreasing C-reactive protein, signifying promising therapeutic potential. Highlighting interactions between top-scoring phytoligands (epicatechin, catechin, and kaempferol) and COX-2, the findings underscore their drug-like characteristics, favorable pharmacokinetics, and enhanced safety toxicity profiles. Results from in vitro, in vivo , and in silico studies, highlights CPEE remarkable antioxidant and anti-inflammatory potentials. Competing Interests: * None of the authors of this paper has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of the paper. * It is to specifically state that "No Competing interests are at stake and there is No Conflict of Interest" with other people or organizations that could inappropriately influence or bias the content of the paper. (© 2024 The Authors.)
Databáze:	MEDLINE
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