A cohesive Microcoleus strain cluster causes benthic cyanotoxic blooms in rivers worldwide.

Autor:	Junier P; Laboratory of Microbiology, University of Neuchâtel, Switzerland., Cailleau G; Laboratory of Microbiology, University of Neuchâtel, Switzerland., Fatton M; Laboratory of Microbiology, University of Neuchâtel, Switzerland., Udriet P; Laboratory of Microbiology, University of Neuchâtel, Switzerland., Hashmi I; Laboratory of Microbiology, University of Neuchâtel, Switzerland., Bregnard D; Laboratory of Microbiology, University of Neuchâtel, Switzerland., Corona-Ramirez A; Laboratory of Microbiology, University of Neuchâtel, Switzerland., Francesco ED; Laboratory of Microbiology, University of Neuchâtel, Switzerland., Kuhn T; Laboratory of Microbiology, University of Neuchâtel, Switzerland., Mangia N; Laboratory of Microbiology, University of Neuchâtel, Switzerland., Zhioua S; Laboratory of Microbiology, University of Neuchâtel, Switzerland., Hunkeler D; Centre for Hydrogeology and Geothermics, University of Neuchâtel, Switzerland., Bindschedler S; Laboratory of Microbiology, University of Neuchâtel, Switzerland., Sieber S; Department of Chemistry, University of Zürich, Switzerland., Gonzalez D; Laboratory of Microbiology, University of Neuchâtel, Switzerland.
Jazyk:	angličtina
Zdroj:	Water research X [Water Res X] 2024 Sep 04; Vol. 24, pp. 100252. Date of Electronic Publication: 2024 Sep 04 (Print Publication: 2024).
DOI:	10.1016/j.wroa.2024.100252
Abstrakt:	Over the last two decades, proliferations of benthic cyanobacteria producing derivatives of anatoxin-a have been reported in rivers worldwide. Here, we follow up on such a toxigenic event happening in the Areuse river in Switzerland and investigate the diversity and genomics of major bloom-forming riverine benthic cyanobacteria. We show, using 16S rRNA-based community profiling, that benthic communities are dominated by Oscillatoriales. We correlate the detection of one Microcoleus sequence variant matching the Microcoleus anatoxicus species with the presence of anatoxin-a derivatives and use long-read metagenomics to assemble complete circular genomes of the strain. The main dihydro-anatoxin-a-producing strain in the Areuse is distinct from strains isolated in New Zealand, the USA, and Canada, but forms a monophyletic strain cluster with them with average nucleotide identity values close to the species threshold. Compared to the rest of the Microcoleus genus, the toxin-producing strains encode a 15 % smaller genome, lacking genes for the synthesis of some essential vitamins. Toxigenic mats harbor a distinct microbiome dominated by proteobacteria and bacteroidetes, which may support cyanobacterial growth by providing them with essential nutrients. We recommend that strains closely related to M. anatoxicus be monitored internationally in order to help predict and mitigate similar cyanotoxic events. Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Diego Gonzalez reports financial support was provided by Swiss National Science Foundation. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Author(s).)
Databáze:	MEDLINE
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