Circulating proteomic profiles in women with morbid obesity compared to normal-weight women.

Autor: Bertran L; Departament de Medicina i Cirurgia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili (URV), 43201 Reus, Spain. Electronic address: laia.bertran@fundacio.urv.cat., Rusu EC; GEMMAIR Research Group, Institut d'Investigació Sanitària Pere Virgili (IISPV), 43005 Tarragona, Spain. Electronic address: elena.rusu@iispv.cat., Guirro M; Centre for Omic Sciences (COS), Joint Unit URV-EURECAT, Unique Scientific and Technical Infrastructures (ICTS), Eurecat, Centre Tecnològic de Catalunya, 43204 Reus, Spain. Electronic address: maria.guirro@eurecat.cat., Aguilar C; Departament de Medicina i Cirurgia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili (URV), 43201 Reus, Spain; GEMMAIR Research Group, Institut d'Investigació Sanitària Pere Virgili (IISPV), 43005 Tarragona, Spain. Electronic address: carmenisabel.aguilar@urv.cat., Auguet T; Departament de Medicina i Cirurgia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili (URV), 43201 Reus, Spain; GEMMAIR Research Group, Institut d'Investigació Sanitària Pere Virgili (IISPV), 43005 Tarragona, Spain. Electronic address: mariateresa.auguet@urv.cat., Richart C; Departament de Medicina i Cirurgia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili (URV), 43201 Reus, Spain. Electronic address: crichartjurado@gmail.com.
Jazyk: angličtina
Zdroj: Journal of proteomics [J Proteomics] 2025 Jan 06; Vol. 310, pp. 105317. Date of Electronic Publication: 2024 Sep 21.
DOI: 10.1016/j.jprot.2024.105317
Abstrakt: In this study, we aimed to evaluate circulating proteomic levels in women with morbid obesity (MO) compared to normal-weight (NW) women. Moreover, we have compared the proteomic profile between women with metabolically healthy (MH) MO and those with type 2 diabetes mellitus (T2DM). The study included 66 normal-weight (NW) women and 129 women with MO (54 MH and 75 with T2DM). Blood samples were processed for proteomics, involving protein extraction, quantification, digestion with peptide labelling and Nano (liquid chromatography (LC)-(Orbitrap) coupled to mass/mass spectrometry (MS/MS) analysis. Statistical analyses were performed. We identified 257 proteins. Women with MO showed significantly increased levels of 35 proteins and decreased levels of 45 proteins compared to NW women. Enrichment analysis of metabolic pathways revealed significant findings. Women with MO have an altered proteomic profile compared to normal-weight women, involving proteins significantly related to chylomicron assembly, complement cascade, clotting pathways and the insulin growth factor system. Regarding women with MO and T2DM compared to MHMO women, the proteomic profile shows alterations in mostly the same pathways associated with obesity. These findings confirmed in previous reports can help us better understand the pathophysiology of obesity and associated diseases. SIGNIFICANCE: Women with morbid obesity (MO) exhibit substantial proteomic alterations compared to normal-weight (NW) women, involving 80 proteins. These alterations are linked to significant metabolic pathways, including chylomicron assembly, complement cascade, clotting pathways and the insulin growth factor system. Additionally, women with MO and type 2 diabetes mellitus (T2DM) compared to metabolically healthy MO women share similar proteomic changes than the first comparison. These findings enhance our understanding of the pathophysiology of obesity and associated diseases, offering potential targets for therapeutic intervention.
Competing Interests: Declaration of competing interest Laia Bertran declares no conflicts of interest. Elena Cristina Rusu declares no conflicts of interest. Maria Guirro declares no conflicts of interest. Carmen Aguilar declares no conflicts of interest. Teresa Auguet declares no conflicts of interest. Cristóbal Richart declares no conflicts of interest.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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