Reducing amplification cycles to improve the coverage of influenza A virus genome sequencing in heterosubtypic co-infection.

Autor: Zhang Y; Department of Pathogen, Wuhan Centers for Disease Prevention and Control, Wuhan 430024, China. Electronic address: zhangyijie@whcdc.org., Kong W; Department of Pathogen, Wuhan Centers for Disease Prevention and Control, Wuhan 430024, China. Electronic address: 429466621@qq.com., Wu Y; Department of Pathogen, Wuhan Centers for Disease Prevention and Control, Wuhan 430024, China. Electronic address: wuyixuan@whcdc.org., Chen Z; Department of Pathogen, Wuhan Centers for Disease Prevention and Control, Wuhan 430024, China. Electronic address: 694867557@qq.com., Zhao X; National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. Electronic address: zhaoxiang@ivdc.chinacdc.cn., Liu M; Department of Pathogen, Wuhan Centers for Disease Prevention and Control, Wuhan 430024, China. Electronic address: liumq33@hotmail.com.
Jazyk: angličtina
Zdroj: Journal of virological methods [J Virol Methods] 2024 Dec; Vol. 330, pp. 115036. Date of Electronic Publication: 2024 Sep 20.
DOI: 10.1016/j.jviromet.2024.115036
Abstrakt: This study delineates the enhancement of a Reverse Transcription Polymerase Chain Reaction (RT-PCR) method for the amplification of the complete genome of the influenza A virus during heterosubtypic co-infection, relying on the amplification of intact gene segments. The precision of the method was assessed using all amplicons, which underwent both capillary electrophoresis and DNA sequencing. Five samples featuring co-infection of Influenza A viruses with H1N1 and H3N2 subtypes were evaluated. The improved strategy successfully amplified all eight segments of H3N2 strains in four samples, and the entire genome of H1N1 strains in three samples.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interestsor personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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