Characterization of Tfgal-9: A galectin in innate immune system of Trachidermus fasciatus - Insights into its sequence analysis, expression patterns, and in vitro bioactivities.

Autor: Xu Z; SDU-ANU Joint Science College, Shandong University, Weihai, 264209, China; Department of Ocean Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, 999077, China. Electronic address: zxudk@connect.ust.hk., Yu S; Marine College, Shandong University, Weihai, Shandong, 264209, China. Electronic address: yushanshan@sdu.edu.cn., Xu C; Department of Sociology, Zhejiang University, Hangzhou, 310058, China; Market Supervision Bureau of Nanxun District, Huzhou, 313009, China., Zhao J; SDU-ANU Joint Science College, Shandong University, Weihai, 264209, China; Mellon College of Science, Carnegie Mellon University, Fifth Avenue, Pittsburgh, PA, 15213, USA. Electronic address: jiayizh3@andrew.cmu.edu., Zhu J; SDU-ANU Joint Science College, Shandong University, Weihai, 264209, China. Electronic address: 202000700248@mail.sdu.edu.cn., Liu D; SDU-ANU Joint Science College, Shandong University, Weihai, 264209, China; Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, China. Electronic address: liu_dun@mail.ustc.edu.cn., Peng M; Department of Biology, McGill University, 845 Rue Sherbrooke O, Montréal, QC, H3A 0G4, Canada. Electronic address: min.peng@mail.mcgill.ca., Liu Y; Marine College, Shandong University, Weihai, Shandong, 264209, China. Electronic address: liuying0631@sdu.edu.cn., Zhu Q; Marine College, Shandong University, Weihai, Shandong, 264209, China. Electronic address: qianzhu@sdu.edu.cn.
Jazyk: angličtina
Zdroj: Fish & shellfish immunology [Fish Shellfish Immunol] 2024 Nov; Vol. 154, pp. 109915. Date of Electronic Publication: 2024 Sep 19.
DOI: 10.1016/j.fsi.2024.109915
Abstrakt: An in-depth understanding of the immune system of endangered species is crucial for successful conservation efforts. Galectins, as members of the lectin family, play a crucial role in the fish innate immune system. Galectin-9 (Tfgal-9) was cloned from endangered species Trachidermus fasciatus, revealing a cDNA sequence of 1453 bp with an open reading frame of 900 bp encoding a protein of 299 amino acids. Tfgal-9 protein features two repeated carbohydrate-binding domains, each characterized by two conserved galactose-binding sites (H-NPR and WG-EER), and it possesses neither a signal peptide nor a transmembrane domain. The qRT-PCR analysis revealed that Tfgal-9 was widely expressed across all examined tissues, with the highest expression in the intestine, followed by the blood, heart and brain. Expression was notably up-regulated in the blood, skin, liver, stomach, and heart when challenged with LPS. Following induction by the heavy metal solution containing Cu, Pb, Cd, and Hg, the expression Tfgal-9 was dramatically induced to 32 times higher than that of the control group in the brain. The recombinant Tfgal-9 protein exhibits calcium-independent binding and agglutination of selected bacteria and yeast. Antimicrobial activity of recombinant Tfgal-9 protein against Gram positive bacteria Staphylococcus aureus was confirmed using the cylinder-plate method. In vitro antioxidant experiments showed that radical scavenging activity of DPPH was 50.38 % when Tfgal-9 concentration reached 200 μg/mL. These results indicate that Tfgal-9 may play important roles in the immune response against microbial infections and the maintaining of redox homeostasis.
Competing Interests: Declaration of competing interest The authors hereby declare no completing interests.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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