Structural identification and immunostimulatory effect of Bacillus velezensis GV1 polysaccharides via TLR4/NF-κB signaling pathway in RAW264.7 macrophages.

Autor: Le MH; Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, Republic of Korea. Electronic address: lehaminh2311@khu.ac.kr., Humayun S; School of Natural Sciences and Health, Tallinn University, Narva mantee 25, 10120 Tallinn, Estonia. Electronic address: sanjida@tlu.ee., Lee HJ; Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, Republic of Korea. Electronic address: gy9707@khu.ac.kr., Mi XJ; School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, Anhui, China. Electronic address: xjm_1207@ahtcm.edu.cn., Justine EE; Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, Republic of Korea. Electronic address: elsaeaster96@khu.ac.kr., Tran THM; Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, Republic of Korea. Electronic address: hoamytran96@khu.ac.kr., Park HR; Department of Hotel Food-service & Culinary Arts, Suwon Women's University, 1098 Juweok-ro, Hwaseong-si, Gyeonggi-do 18333, Republic of Korea. Electronic address: hrpark@swc.ac.kr., Kim YJ; Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, Republic of Korea. Electronic address: yeonjukim@khu.ac.kr.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Nov; Vol. 280 (Pt 2), pp. 135808. Date of Electronic Publication: 2024 Sep 19.
DOI: 10.1016/j.ijbiomac.2024.135808
Abstrakt: Microbial polysaccharides derived from bacterial sources possess unique properties because of their structural complexity contributing to exceptional characteristics, including potent immunostimulatory effects. In this study, we extracted crude polysaccharide from Bacillus velezensis GV1 (BPS) which was isolated from Korean ginseng vinegar, and subsequently characterized for sugar composition and functional groups using FT-IR and methylation method. Structural analysis indicated that BPS was composed of mannan and glucan in a ratio of 7.5:2.5. The immunostimulatory effect of BPS was investigated in RAW264.7 macrophages. The results revealed that BPS significantly increased NO production, as well as the secretion and expression of key cytokines, such as IL-6, TNF-α, and IL-1β. These effects were confirmed using a TLR4 antagonist (TAK-242). Moreover, BPS exhibited immunostimulatory potential by promoting the NF-κB signaling pathway. In conclusion, this study establishes a foundation for the potential application of BPS as an immunostimulatory adjuvant or alternative component in functional foods, particularly for enhancing innate immune responses.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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