VrNIN1 interacts with VrNNC1 to regulate root nodulation in mungbean.

Autor: Zhang Y; College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China., Hou R; College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China., Yao X; College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China., Wang X; College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China., Li W; College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China., Fang X; College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China., Ma X; College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China., Li S; College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China. Electronic address: li2014shuai@qau.edu.cn.
Jazyk: angličtina
Zdroj: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2024 Nov; Vol. 216, pp. 109131. Date of Electronic Publication: 2024 Sep 18.
DOI: 10.1016/j.plaphy.2024.109131
Abstrakt: Node Inception (NIN) plays a crucial role in legume symbiosis by participating in both infection and nodule formation processes. However, its specific function in mungbean (Vigna radiata) remains poorly understood. This study aimed to functionally characterize the VrNIN1 gene in mungbean through an enhanced hairy root transformation approach. Examination of proVrNIN1: GUS hairy roots via GUS staining indicated the expression of VrNIN1 in later root promodia, nodule primordia, and nodules. Phenotypic evaluation revealed that overexpression or silencing of VrNIN1 led to a significant reduction in nodule numbers in hairy roots compared to controls. Additionally, interaction between VrNIN1 and VrNNC1 was confirmed through yeast two-hybrid, luciferase complementation and Co-immunoprecipitation assays. VrNNC1 expression was observed in the vascular bundle and cortex of roots and root nodules, where it notably suppressed nodule formation in transgenic hairy roots. Furthermore, gene expression analysis demonstrated the involvement of VrNIN1 and VrNNC1 in regulating root nodulation by modulating the expression of VrRIC1 and VrEDOD40. This study not only optimized the genetic transformation system for hairy roots in mungbean, but also provided mechanistic insights into the regulatory role of VrNIN1 in root nodule symbiosis in mungbean.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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Databáze: MEDLINE
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