18 F-fluorodeoxyglucose PET-MR characterization of aortic inflammation in ApoE -/- mouse models of accelerated atherosclerosis: comparison of Western diet vs. uremia.

Autor: Deshayes S; Department of Internal Medicine, Normandie Univ, UNICAEN, CHU de Caen Normandie, Caen, 14000, France.; Normandie Univ, UNICAEN, UR4650 PSIR, CHU de Caen Normandie, Caen, 14000, France., Ruello P; Normandie Univ, UNICAEN, UR4650 PSIR, CHU de Caen Normandie, Caen, 14000, France., Simard C; Normandie Univ, UNICAEN, UR4650 PSIR, CHU de Caen Normandie, Caen, 14000, France., Dupont PA; Normandie Univ, UNICAEN, UR4650 PSIR, CHU de Caen Normandie, Caen, 14000, France., Bauge C; Normandie Univ, UNICAEN, UR4650 PSIR, CHU de Caen Normandie, Caen, 14000, France., Abbas A; Normandie Univ, UNICAEN, UR4650 PSIR, CHU de Caen Normandie, Caen, 14000, France., de Boysson H; Department of Internal Medicine, Normandie Univ, UNICAEN, CHU de Caen Normandie, Caen, 14000, France.; Normandie Univ, UNICAEN, UR4650 PSIR, CHU de Caen Normandie, Caen, 14000, France., Aouba A; Department of Internal Medicine, Normandie Univ, UNICAEN, CHU de Caen Normandie, Caen, 14000, France., Manrique A; Normandie Univ, UNICAEN, UR4650 PSIR, CHU de Caen Normandie, Caen, 14000, France. manrique@cyceron.fr.; Department of Nuclear Medicine, Normandie Univ, UNICAEN, CHU de Caen Normandie, Caen, 14000, France. manrique@cyceron.fr.; UR4650 PSIR, GIP Cyceron, Campus Jules Horowitz, Boulevard Henri Becquerel, BP 5229, Caen, 14074, France. manrique@cyceron.fr.
Jazyk: angličtina
Zdroj: The international journal of cardiovascular imaging [Int J Cardiovasc Imaging] 2024 Sep 21. Date of Electronic Publication: 2024 Sep 21.
DOI: 10.1007/s10554-024-03238-0
Abstrakt: ApoE -/- mice are a widely used preclinical model of atherosclerosis, potentially accelerated by a Western diet (WD) or uremia. We aimed to compare hybrid 18 F-fluorodeoxyglucose ( 18 F-FDG) positron emission tomography-magnetic resonance (PET-MR) and immunostaining in ApoE -/- models of accelerated atherosclerosis. Five groups were studied: standard diet-fed ApoE -/- (n = 7), standard diet-fed and uremic ApoE -/- (n = 7), WD ApoE -/- (n = 7), WD and uremic ApoE -/- (n = 6), and control C57BL/6J mice (n = 6). Uremia was induced by electrocoagulation of the right kidney at 8 weeks old, followed 2 weeks later by a contralateral nephrectomy. 18 F-FDG PET-MR imaging and histological staining (anti-CD4, -CD8, -CD11c, -CD20, -CD31, -CD68, -CD163, -interferon-γ, interleukin-1α, -1β, -6, -17 A antibodies) were performed in 18-week-old mice, i.e., 8 weeks after 5/6 nephrectomy and/or WD.  18 F-FDG uptake was similar in all groups. In contrast, histological staining highlighted higher percentages of CD8-, CD68-, or CD11c-positive cells in ApoE -/- aortic samples than in wild-type aortic samples. In addition, immunostaining revealed some differences between ApoE -/- mouse groups. Only the WD seemed to contribute to these differences. Using immunostaining, WD appeared to be a stronger accelerator of atherosclerosis than uremia. However, 18 F-FDG PET-MR imaging failed to demonstrate in vivo increased aortic glucose uptake in these models.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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