Streptococcus spp. isolated from bovine mastitis: Antimicrobial sensitivity studies and disagreement evaluation between routine phenotypic diagnosis and molecular identification.

Autor:	Diana L; Laboratorio de Microbiología, Departamento de Patobiología, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay. Electronic address: leticia.diana@fvet.edu.uy., Mastroianni L; Laboratorio de Microbiología, Departamento de Patobiología, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay., Diana V; Laboratorio de la Rural de San José, San José, Uruguay., Puentes R; Laboratorio de Microbiología, Departamento de Patobiología, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
Jazyk:	angličtina
Zdroj:	Revista Argentina de microbiologia [Rev Argent Microbiol] 2024 Oct-Dec; Vol. 56 (4), pp. 351-358. Date of Electronic Publication: 2024 Sep 19.
DOI:	10.1016/j.ram.2024.07.006
Abstrakt:	Bovine mastitis poses a significant threat to global dairy production, resulting in substantial losses in milk production. Streptococcus bacteria, particularly Streptococcus uberis, Streptococcus agalactiae, and Streptococcus dysgalactiae, are commonly implicated in this condition. An accurate diagnosis is crucial for implementing effective treatment and minimizing its impact on production. This study examined 115 Streptococcus strains isolated from bovine mastitis cases in Uruguay using PCR for species identification. Additionally, the resistance to tetracycline, erythromycin, and penicillin was assessed in 81 of the bacterial strains under study. Significant disparities between phenotypic and genotypic detection were evident across all three species, with only 31% of strains identified phenotypically aligning with PCR results. Phenotypic prevalence indicated S. dysgalactiae as the most prevalent (44.35%), followed by S. uberis (24.34%) and S. agalactiae (6.09%). However, the genotypic identification revealed S. uberis as the most prevalent, followed by S. dysgalactiae, while S. agalactiae remained the least prevalent. The high sensitivity and speed of PCR suggest its potential routine implementation for diagnosing bovine mastitis caused by Streptococcus in any laboratory. Although, penicillin resistance was practically nonexistent, tetracycline and erythromycin exhibit higher resistance levels across all three species studied. In conclusion, the study underlines the importance of early diagnosis, highlights variations in bacterial prevalence, and proposes PCR as a valuable diagnostic tool for Streptococcus species responsible for bovine mastitis. (Copyright © 2024 The Author(s). Publicado por Elsevier España, S.L.U. All rights reserved.)
Databáze:	MEDLINE
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