Engineering the mangrove soil microbiome for selection of polyethylene terephthalate-transforming bacterial consortia.

Autor: Jiménez DJ; Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia. Electronic address: diego.jimenezavella@kaust.edu.sa., Chaparro D; Microbiomes and Bioenergy Research Group, Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia; Max Planck Tandem Group in Computational Biology, Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia., Sierra F; Microbiomes and Bioenergy Research Group, Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia; Max Planck Tandem Group in Computational Biology, Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia., Custer GF; Department of Plant Science and Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, USA; The One Health Microbiome Center, Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, USA., Feuerriegel G; Department of Microbiology and Biotechnology, University of Hamburg, Hamburg, Germany., Chuvochina M; The University of Queensland, School of Chemistry and Molecular Biosciences, Australian Centre for Ecogenomics, Brisbane, Queensland, Australia., Diaz-Garcia L; Department of Chemical and Biological Engineering, Advanced Biomanufacturing Centre, University of Sheffield, Sheffield, UK., Mendes LW; Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture, University of Sao Paulo, Piracicaba, SP, Brazil., Ortega Santiago YP; Department of Chemical and Food Engineering, Universidad de los Andes, Bogotá, Colombia; Research Management, Agroindustrial Production and Transformation Research Group (GIPTA), Department of Agroindustrial Sciences, Universidad Popular del Cesar, Aguachica, Cesar, Colombia., Rubiano-Labrador C; Chemical and Biological Studies Group, Basic Sciences Faculty, Universidad Tecnológica de Bolívar, Cartagena de Indias, Colombia., Salcedo Galan F; Department of Chemical and Food Engineering, Universidad de los Andes, Bogotá, Colombia., Streit WR; Department of Microbiology and Biotechnology, University of Hamburg, Hamburg, Germany., Dini-Andreote F; Department of Plant Science and Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, USA; The One Health Microbiome Center, Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, USA., Reyes A; Max Planck Tandem Group in Computational Biology, Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia., Rosado AS; Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia. Electronic address: alexandre.rosado@kaust.edu.sa.
Jazyk: angličtina
Zdroj: Trends in biotechnology [Trends Biotechnol] 2024 Sep 20. Date of Electronic Publication: 2024 Sep 20.
DOI: 10.1016/j.tibtech.2024.08.013
Abstrakt: Mangroves are impacted by multiple environmental stressors, including sea level rise, erosion, and plastic pollution. Thus, mangrove soil may be an excellent source of as yet unknown plastic-transforming microorganisms. Here, we assess the impact of polyethylene terephthalate (PET) particles and seawater intrusion on the mangrove soil microbiome and report an enrichment culture experiment to artificially select PET-transforming microbial consortia. The analysis of metagenome-assembled genomes of two bacterial consortia revealed that PET catabolism can be performed by multiple taxa, of which particular species harbored putative novel PET-active hydrolases. A key member of these consortia (Mangrovimarina plasticivorans gen. nov., sp. nov.) was found to contain two genes encoding monohydroxyethyl terephthalate hydrolases. This study provides insights into the development of strategies for harnessing soil microbiomes, thereby advancing our understanding of the ecology and enzymology involved in microbial-mediated PET transformations in marine-associated systems.
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: