Cunninghamella echinulata DSM1905 biofilm-based L-asparaginase production in pneumatically-driven bioreactors.

Autor: Ramos RCPDS; Graduate Program in Dentistry, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil.; Xenobiotics Research Unit, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil., de Oliveira NS; Xenobiotics Research Unit, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil.; Graduate Program in Animal Sciences, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil., Bianchini LF; Xenobiotics Research Unit, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil., Azevedo-Alanis LR; Graduate Program in Dentistry, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil., Pimentel IC; Department of Microbiology, Immunology and Parasitology, Federal University of Paraná, Curitiba, Brazil., Hardy AMTG; College of Pharmacy and Pharmaceutical Sciences, The University of Toledo, Toledo, Ohio, United States of America., Murata RM; The Brody School of Medicine, East Carolina University, Greenville, North Carolina, United States of America., Glassey J; School of Engineering, Newcastle University, Newcastle-upon-Tyne, United Kingdom., Rosa EAR; Graduate Program in Dentistry, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil.; Xenobiotics Research Unit, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil.; Graduate Program in Animal Sciences, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Sep 20; Vol. 19 (9), pp. e0308847. Date of Electronic Publication: 2024 Sep 20 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0308847
Abstrakt: We evaluated by comparing the performance of three pneumatically-driven bioreactors in the production of L-asparaginase (L-ASNase), an enzyme used to treat leukaemia and lymphoma. A two-step screening process was conducted to detect Cunninghamella spp. strains producing L-ASNase. Cunninghamella echinulata DSM1905 produced the highest levels of L-ASNase during screening assays. Subsequently, fermentations were performed in bubble column (BCR), airlift (ALR), and hybrid fixed-bed airlift (FB-ALR) bioreactors to determine the best upstream bioprocess. Mycelial biomass production was higher in BCR than in ALR and FB-ALR (p ≤ 0.0322). The activity of L-ASNase produced in FB-ALR, in which the fungus grew as a consistent biofilm, was significantly higher (p ≤ 0.022) than that from ALR, which was higher than that of BCR (p = 0.036). The specific activity of ALR and FB-ALR presented no differences (p = 0.073), but it was higher than that of BCR (p ≤ 0.032). In conclusion, C. echinulata DSM1905, grown under the biofilm phenotype, produced the highest levels of L-ASNase, and FB-ALR was the best upstream system for enzyme production.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Ramos et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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