CD11b/CD86 involved in the microenvironment of colorectal cancer by promoting Wnt signaling activation.

Autor: Ke J; School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.; Gaozhou Hospital of Traditional Chinese Medicine Affiliated to Guangzhou University of Chinese Medicine, Gaozhou, China., Chen G; Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China., You Y; Maoming Hospital of Traditional Chinese Medicine Affiliated to Guangzhou University of Chinese Medicine, Maoming, China., Xie Q; Animal Experiment Center, Guangzhou University of Chinese Medicine, Guangzhou, China., Liu ZL; The First Clinical Medical College of Guangzhou University of Traditional Chinese Medicine, Guangzhou, China., Song C; Guangzhou International Bio Island, Guangzhou, China., Zheng Y; Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China., Shan Z; School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China., Song J; Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China., Jiang Z; International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China., Wang H; School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China., Du Q; Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China., Wu Y; Gaozhou Hospital of Traditional Chinese Medicine Affiliated to Guangzhou University of Chinese Medicine, Gaozhou, China., Chen XL; School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China., Li Y; Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China.
Jazyk: angličtina
Zdroj: Cancer medicine [Cancer Med] 2024 Sep; Vol. 13 (18), pp. e70245.
DOI: 10.1002/cam4.70245
Abstrakt: Background: Colorectal cancer (CRC) is a malignancy that arises within the gastrointestinal tract. Despite ongoing research, the etiology and pathogenesis of CRC remain elusive; particularly, the distribution and characteristics of tumor-associated macrophages is currently an active area of investigation in understanding the pathological progression and prevention of CRC.
Methods: This study utilized CRC patient surgical samples, mouse models of colitis-associated cancer, colonic organoid, and co-culture cell line to examine the changes in CD11b/CD86 at different pathological region and detect the Wnt signaling pathway activity.
Results: Our findings revealed a sensitive and increased expression of CD11b from the early to the advanced CRC tissues and correlated with poor prognosis, while CD86 expression was reduced in advanced CRC tissues. CD133 expression was also elevated in advanced CRC tissues and mainly co-localized with CD11b, suggesting a positive regulatory effect of CD11b and CD133 expression that may contribute to CRC progression. In AOM/DSS mouse models, activation of the Wnt signaling pathway was associated with increased CD133 and CD11b expression. In vitro, THP-1 cell was induced to high expression of CD11b, and the above conditional cultural medium enhanced HCT116 cell colony number and CD133 protein expression. Furthermore, colonic crypts from AOM/DSS mouse models were isolated to culture, and the colonic organoids exhibited dilation and significant increases expression of CD133 and β-Catenin/N-P-B-Catenin.
Conclusions: CD11b might be an important factor to participate the progress of CRC. And the high CD11b of CRC microenviroment might potentially promote CD133 expression and associate with Wnt signal activation.
(© 2024 The Author(s). Cancer Medicine published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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