Duck hepatitis A virus utilizes PCBP2 to facilitate viral translation and replication.

Autor: Xu C; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China., Jiang Y; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China., Wang M; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China., Cheng A; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China. chenganchun@vip.163.com.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China. chenganchun@vip.163.com.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China. chenganchun@vip.163.com.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China. chenganchun@vip.163.com.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. chenganchun@vip.163.com., Zhang W; Sinopharm Yangzhou VAC Biological Engineering Co., Ltd., Yangzhou, 225100, China., Ou X; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China., Sun D; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China., Yang Q; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China., Wu Y; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China., Tian B; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China., He Y; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China., Wu Z; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China., Zhang S; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China., Zhao X; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China., Huang J; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China., Zhu D; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China., Chen S; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China., Liu M; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China., Jia R; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China.; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China.; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Jazyk: angličtina
Zdroj: Veterinary research [Vet Res] 2024 Sep 19; Vol. 55 (1), pp. 110. Date of Electronic Publication: 2024 Sep 19.
DOI: 10.1186/s13567-024-01369-9
Abstrakt: Duck hepatitis A virus type 1 (DHAV-1) is an important member of the Picornaviridae family that causes highly fatal hepatitis in ducklings. Since picornaviruses have small genomes with limited coding capacity, they must utilize host proteins for viral cap-independent translation and RNA replication. Here, we report the role of duck poly(rC)-binding protein 2 (PCBP2) in regulating the replication and translation of DHAV-1. During DHAV-1 infection, PCBP2 expression was upregulated. A biotinylated RNA pull-down assay revealed that PCBP2 positively regulates DHAV-1 translation through specific interactions with structural domains II and III of the DHAV-1 internal ribosome entry site (IRES). Further studies revealed that PCBP2 promotes DHAV-1 replication via an interaction of its KH1 domain (aa 1-92) with DHAV-1 3D pol . Thus, our studies demonstrated the specific role of PCBP2 in regulating DHAV-1 translation and replication, revealing a novel mechanism by which host‒virus interactions regulate viral translation and replication. These findings contribute to further understanding of the pathogenesis of picornavirus infections.
(© 2024. The Author(s).)
Databáze: MEDLINE
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