Multiscale and multimodal evaluation of autosomal dominant polycystic kidney disease development.

Autor: Delgado-Rodriguez P; Bioengineering Department, Universidad Carlos III de Madrid, Madrid, Spain. pdelgado@pa.uc3m.es.; Instituto de Investigacion Sanitaria Gregorio Marañon (IiSGM), Madrid, Spain. pdelgado@pa.uc3m.es., Lamanna-Rama N; Instituto de Investigacion Sanitaria Gregorio Marañon (IiSGM), Madrid, Spain.; Instituto de Investigacion Sanitaria Fundación Jimenez Diaz (IIS - FJD), Madrid, Spain., Saande C; Division of Gene Therapy and Regulation of Gene Expression, Centre for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain., Aldabe R; Division of Gene Therapy and Regulation of Gene Expression, Centre for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain., Soto-Montenegro ML; Instituto de Investigacion Sanitaria Gregorio Marañon (IiSGM), Madrid, Spain.; CIBER de Salud Mental (CIBERSAM), Madrid, Spain.; High Performance Research Group in Physiopathology and Pharmacology of the Digestive System (NeuGut), University Rey Juan Carlos (URJC), Alcorcon, Spain., Munoz-Barrutia A; Bioengineering Department, Universidad Carlos III de Madrid, Madrid, Spain.; Instituto de Investigacion Sanitaria Gregorio Marañon (IiSGM), Madrid, Spain.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2024 Sep 19; Vol. 7 (1), pp. 1183. Date of Electronic Publication: 2024 Sep 19.
DOI: 10.1038/s42003-024-06868-1
Abstrakt: Autosomal Dominant Polycystic Kidney Disease (ADPKD) is the most prevalent kidney genetic disorder, producing structural abnormalities and impaired function. This research investigates its evolution on mouse models, utilizing a combination of histology imaging, Computed Tomography (CT) and Magnetic Resonance Imaging (MRI) to evaluate its progression thoroughly. ADPKD has been induced in mice via PKD2 gene knockout, followed by image acquisition at different stages. Histology data provides two-dimensional details, like the cystic area ratio, whereas CT and MRI facilitate three-dimensional temporal monitoring. Our approach allows to quantify the affected tissue at different disease stages through multiple quantitative metrics. A pivotal point is shown at approximately ten weeks after induction, marked by a swift acceleration in disease advancement, and leading to a notable increase in cyst formation. This multimodal strategy augments our comprehension of ADPKD dynamics and suggests the possibility of employing higher-resolution imaging in the future for more accurate volumetric analyses.
(© 2024. The Author(s).)
Databáze: MEDLINE
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