Association between O-GlcNAc levels and platelet function in obese insulin-resistant subjects.

Autor: Hernández-Huerta MT; CONAHCyT Facultad de Medicina y Cirugía, Universidad Autónoma 'Benito Juárez' de Oaxaca, Oaxaca, 68020, México. mthernandez@conahcyt.mx., Martínez-Cruz R; Centro de Investigación Facultad de Medicina UNAM-UABJO, Facultad de Medicina y Cirugía, Universidad Autónoma 'Benito Juárez' de Oaxaca, Oaxaca, 68020, México., Pérez-Campos Mayoral L; Centro de Investigación Facultad de Medicina UNAM-UABJO, Facultad de Medicina y Cirugía, Universidad Autónoma 'Benito Juárez' de Oaxaca, Oaxaca, 68020, México., Pina-Canseco MDS; Centro de Investigación Facultad de Medicina UNAM-UABJO, Facultad de Medicina y Cirugía, Universidad Autónoma 'Benito Juárez' de Oaxaca, Oaxaca, 68020, México., Solórzano-Mata CJ; Centro de Investigación Facultad de Medicina UNAM-UABJO, Facultad de Medicina y Cirugía, Universidad Autónoma 'Benito Juárez' de Oaxaca, Oaxaca, 68020, México.; Facultad de Odontología, Universidad Autónoma 'Benito Juárez' de Oaxaca, Oaxaca City, 68120, México., Martínez-Cruz M; Tecnológico Nacional de México/IT de Oaxaca, Oaxaca, 68030, México., Vásquez Martínez IP; Centro de Investigación Facultad de Medicina UNAM-UABJO, Facultad de Medicina y Cirugía, Universidad Autónoma 'Benito Juárez' de Oaxaca, Oaxaca, 68020, México., Zenteno E; Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México, 04360, México., Laguna Barrios LÁ; Centro de Investigación Facultad de Medicina UNAM-UABJO, Facultad de Medicina y Cirugía, Universidad Autónoma 'Benito Juárez' de Oaxaca, Oaxaca, 68020, México., Matias-Cervantes CA; CONAHCyT Facultad de Medicina y Cirugía, Universidad Autónoma 'Benito Juárez' de Oaxaca, Oaxaca, 68020, México., Pérez-Campos Mayoral E; Centro de Investigación Facultad de Medicina UNAM-UABJO, Facultad de Medicina y Cirugía, Universidad Autónoma 'Benito Juárez' de Oaxaca, Oaxaca, 68020, México., Pérez-Campos E; Tecnológico Nacional de México/IT de Oaxaca, Oaxaca, 68030, México. perezcampos@prodigy.net.mx.; Laboratorio de Patología Clínica, 'Dr. Eduardo Pérez Ortega,', Oaxaca, 68000, México. perezcampos@prodigy.net.mx.
Jazyk: angličtina
Zdroj: Glycoconjugate journal [Glycoconj J] 2024 Oct; Vol. 41 (4-5), pp. 291-300. Date of Electronic Publication: 2024 Sep 20.
DOI: 10.1007/s10719-024-10164-9
Abstrakt: Obesity is an epidemic associated with platelet and vascular disorders. Platelet O-GlcNAcylation has been poorly studied in obese subjects. We aimed to evaluate O-linked N-acetyl-glucosamine (O-GlcNAc) levels and platelet activity in obese insulin-resistant (ObIR) subjects. Six healthy and six insulin-resistant obese subjects with a body mass index of 22.6 kg/m 2 (SD ± 2.2) and 35.6 kg/m 2 (SD ± 3.8), respectively, were included. Flow cytometry was used to measure markers of platelet activity, expression of P-selectin (CD62P antibody), glycoprotein IIb/IIIa (integrins αIIbβ3 binding to PAC-1 antibody), and thrombin stimulation. O-GlcNAc was determined in the platelets of all test subjects by cytofluometry, intracellular calcium, percentage of platelet aggregation, and immunofluorescence microscopy and Western blot were used to assess O-GlcNAc and OGT (O-GlcNAc transferase) in platelets. Platelets from ObIR subjects had on average 221.4 nM intracellular calcium, 81.89% PAC-1, 22.85% CD62P, 57.48% OGT, and 66.62% O-GlcNAc, while platelets from healthy subjects had on average 719.2 nM intracellular calcium, 4.99% PAC-1, 3.17% CD62P, 18.38% OGT, and 23.41% O-GlcNAc. ObIR subjects showed lower platelet aggregation than healthy subjects, 13.83% and 54%, respectively. The results show that ObIR subjects have increased O-GlcNAc, and increased intraplatelet calcium associated with platelet hyperactivity and compared to healthy subjects, suggesting that changes in platelet protein O-GlcNAcylation and platelet activity might serve as a possible prognostic tool for insulin resistance, prediabetes and its progression to type 2 diabetes mellitus.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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