In vitro toxicity assessment of bioavailable iron in coal varieties of Central India.

Autor: Jain RK; Department of Environmental Science and Engineering, Indian Institute of Technology (Indian School of Mines), Dhanbad, Jharkhand, India.; Department of Biochemistry, National Institute of Miners' Health, Nagpur, Maharashtra, India., Anshumali; Department of Environmental Science and Engineering, Indian Institute of Technology (Indian School of Mines), Dhanbad, Jharkhand, India., Sherekar P; Department of Biotechnology, Priyadarshini College of Engineering, Nagpur, Maharashtra, India., Nayak A; Research Laboratory, G.M. Taori, Central India Institute of Medical Sciences (CIIMS), Nagpur, Maharashtra, India., Jaiswal S; Department of Biochemistry, National Institute of Miners' Health, Nagpur, Maharashtra, India., Pimpalghare K; Research Laboratory, G.M. Taori, Central India Institute of Medical Sciences (CIIMS), Nagpur, Maharashtra, India., Tumane R; Department of Biochemistry, National Institute of Miners' Health, Nagpur, Maharashtra, India.; Department of Biochemistry, Regional Occupational Health Centre (Southern), Bengaluru, Karnataka, India., Jawade A; Department of Biochemistry, National Institute of Miners' Health, Nagpur, Maharashtra, India., Pingle SK; Department of Biochemistry, National Institute of Miners' Health, Nagpur, Maharashtra, India.; Department of Biochemistry, Regional Occupational Health Centre (Southern), Bengaluru, Karnataka, India., Suke SG; Department of Biotechnology, Priyadarshini College of Engineering, Nagpur, Maharashtra, India., Kashyap RS; Research Laboratory, G.M. Taori, Central India Institute of Medical Sciences (CIIMS), Nagpur, Maharashtra, India., Mandal BB; Department of Mining Engineering, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, West Bengal, India.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Sep 19; Vol. 19 (9), pp. e0309237. Date of Electronic Publication: 2024 Sep 19 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0309237
Abstrakt: Introduction: Information on bioavailable Iron (BAI) content in respirable coal dust (RCD) is crucial to address occupational health and safety, especially in preventing coal workers' pneumoconiosis (CWP).
Materials and Methods: In the present study, we determined BAI concentrations in seventy-seven coal samples collected from ten coal mining regions of Central India. The cytotoxic potential of BAI-RCD was established invitro by using alveolar epithelial (A549) and macrophage (U937) cell lines. The oxidative/antioxidant status, inflammations, and genotoxicity attributed to BAI-RCD exposure were evaluated and correlated with CWP pathophysiology.
Results: The mean BAI concentrations in the coal samples (n = 77) range from (275 to 9065 mg kg-1) and showed wide variability. Both cell lines were exposed to low (275 mg kg-1), moderate (4650 mg kg-1), and high (9065 mg kg-1) BAI-RCD samples showed significant (p < 0.001) cytotoxicity in a dose-dependent manner (low < moderate < high) compared to the control. After BAI-RCD treatment, both cell lines showed a decrease in antioxidant stress measures (SOD, CAT, and GSH) and a significant (p < 0.001) increase in oxidative stress parameters (NADPH, MPO, LPO, and PC). Furthermore, these cell line models demonstrated a statistically significant (p < 0.001) dose-dependent increase in cytokines (TGF-β1, IL-1β, TNF-α, MCP-1, and IL-6 cytokines) and oxidative DNA damage marker (8-OH-dG).
Conclusion: Results indicated that the central India coals (even at low BAI content) may be accountable for inflammatory responses and cytotoxicity. Hence, BAI can be important characteristic to establish safety standards for coal dust exposure before active mining.
Competing Interests: The authors have declared that no competing interests exit.
(Copyright: © 2024 Jain et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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