Protocol for semiautomated analysis of sensory neuronal innervation of the mouse footpad skin.
| Autor: | Dey S; Department of Biomolecular Sciences and Molecular Neuroscience, Weizmann Institute of Science, Rehovot 76100, Israel. Electronic address: swagata.dey@mssm.edu., Kovalenko A; Department of Biomolecular Sciences and Molecular Neuroscience, Weizmann Institute of Science, Rehovot 76100, Israel., Yaron A; Department of Biomolecular Sciences and Molecular Neuroscience, Weizmann Institute of Science, Rehovot 76100, Israel. Electronic address: avraham.yaron@weizmann.ac.il. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2024 Dec 20; Vol. 5 (4), pp. 103312. Date of Electronic Publication: 2024 Sep 18. |
| DOI: | 10.1016/j.xpro.2024.103312 |
| Abstrakt: | Here, we present a protocol for staining murine skin innervation by either a pan-axonal marker or a genetic tracer of sensory neuron subtypes using floating sections. We also describe steps for using a new MATLAB-based semiautomated routine that facilitates the quantification of innervation density. This protocol can also be applied to other organs, such as the mouse's spinal cord and tongue. For complete details on the use and execution of this protocol, please refer to Dey et al. 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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