Unveiling novel insights: geraniol's enhanced anti-candida efficacy and mechanistic innovations against multidrug-resistant candida strains.

Autor: da Silva LJ; School of Pharmacy, Laboratory for Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Center for Drug Research and Development (NPDM), Federal University of Ceará, Fortaleza, CE, Brazil., Rodrigues DS; School of Pharmacy, Laboratory for Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Center for Drug Research and Development (NPDM), Federal University of Ceará, Fortaleza, CE, Brazil.; Christus University Center (UNICHRISTUS), Fortaleza, CE, Brazil., de Farias Cabral VP; School of Pharmacy, Laboratory for Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Center for Drug Research and Development (NPDM), Federal University of Ceará, Fortaleza, CE, Brazil., da Silva CR; School of Pharmacy, Laboratory for Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Center for Drug Research and Development (NPDM), Federal University of Ceará, Fortaleza, CE, Brazil., Sá LGDAV; School of Pharmacy, Laboratory for Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Center for Drug Research and Development (NPDM), Federal University of Ceará, Fortaleza, CE, Brazil.; Christus University Center (UNICHRISTUS), Fortaleza, CE, Brazil., de Andrade-Neto JB; School of Pharmacy, Laboratory for Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Center for Drug Research and Development (NPDM), Federal University of Ceará, Fortaleza, CE, Brazil.; Christus University Center (UNICHRISTUS), Fortaleza, CE, Brazil., Barbosa AD; School of Pharmacy, Laboratory for Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Center for Drug Research and Development (NPDM), Federal University of Ceará, Fortaleza, CE, Brazil., Flaresso AA; Christus University Center (UNICHRISTUS), Fortaleza, CE, Brazil., Rocha SNCD; Christus University Center (UNICHRISTUS), Fortaleza, CE, Brazil., Cavalcanti BC; Center for Drug Research and Development (NPDM), Federal University of Ceará, Fortaleza, CE, Brazil.; Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil., Moraes MO; Center for Drug Research and Development (NPDM), Federal University of Ceará, Fortaleza, CE, Brazil.; Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil., Rios MEF; Center for Drug Research and Development (NPDM), Federal University of Ceará, Fortaleza, CE, Brazil.; Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil., Pampolha Filho IS; Department of Pharmacology, School of Medicine, Federal University of Ceará, Barbalha, CE, Brazil., Júnior HVN; School of Pharmacy, Laboratory for Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil. label_ufc@yahoo.com.br.; Center for Drug Research and Development (NPDM), Federal University of Ceará, Fortaleza, CE, Brazil. label_ufc@yahoo.com.br.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2024 Sep 19. Date of Electronic Publication: 2024 Sep 19.
DOI: 10.1007/s42770-024-01498-5
Abstrakt: Objectives: This study addressed the need for new treatments for severe Candida infections, especially resistant strains. It evaluated the antifungal potential of geraniol alone and with fluconazole against various Candida spp., including resistant strains, and investigated geraniol's mechanism of action using flow cytometry.
Methods: The research assessed the inhibitory effects of geraniol on the growth of various Candida species at concentrations ranging from 110 to 883 µg/ml. The study also explored the potential synergistic effects when geraniol was combined with fluconazole. The mechanism of action was investigated through flow cytometry, with a particular emphasis on key enzymes associated with plasma membrane synthesis, membrane permeability changes, mitochondrial membrane depolarization, reactive oxygen species (ROS) induction, and genotoxicity.
Results: Geraniol demonstrated significant antifungal activity against different Candida species, inhibiting growth at concentrations within the range of 110 to 883 µg/ml. The mechanism of action appeared to be multifactorial. Geraniol was associated with the inhibition of crucial enzymes involved in plasma membrane synthesis, increased membrane permeability, induction of mitochondrial membrane depolarization, elevated ROS levels, and the presence of genotoxicity. These effects collectively contributed to cell apoptosis.
Conclusions: Geraniol, alone and in combination with fluconazole, shows promise as a potential therapeutic option for Candida spp.
Infections: Its diverse mechanism of action, impacting crucial cellular processes, highlights its potential as an effective antifungal agent. Further research into geraniol's therapeutic applications may aid in developing innovative strategies to address Candida infections, especially those resistant to current therapies.
(© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
Externí odkaz: