Extending the post-thaw shelf-life of cryoprecipitate when stored at refrigerated temperatures.

Autor: Winter KM; Research and Development, Australian Red Cross Lifeblood, Alexandria, New South Wales, Australia., Webb RG; Research and Development, Australian Red Cross Lifeblood, Alexandria, New South Wales, Australia., Mazur E; Research and Development, Australian Red Cross Lifeblood, Alexandria, New South Wales, Australia., Dennington PM; Pathology Services, Australian Red Cross Lifeblood, Alexandria, New South Wales, Australia., Marks DC; Research and Development, Australian Red Cross Lifeblood, Alexandria, New South Wales, Australia.; Sydney Medical School, The University of Sydney, Camperdown, New South Wales, Australia.
Jazyk: angličtina
Zdroj: Vox sanguinis [Vox Sang] 2024 Dec; Vol. 119 (12), pp. 1257-1267. Date of Electronic Publication: 2024 Sep 18.
DOI: 10.1111/vox.13736
Abstrakt: Background and Objectives: The post-thaw shelf-life of cryoprecipitate is 6 h, leading to high wastage. Storage of thawed cryoprecipitate at refrigerated temperatures may be feasible to extend the shelf-life. This study aimed to evaluate the quality of thawed cryoprecipitate stored at 1-6°C for up to 14 days.
Materials and Methods: Cryoprecipitate (mini- and full-size packs derived from both apheresis and whole blood [WB] collections) was thawed, immediately sampled and then stored at 1-6°C for up to 14 days. Mini-packs were sampled at 6, 24, 48 and 72 h, day 7 and 14; full-size cryoprecipitate was sampled on day 3, 5 or 7. Coagulation factors (F) II, V, VIII, IX, X and XIII, von Willebrand factor (VWF) and fibrinogen were measured using a coagulation analyser. Thrombin generation was measured by calibrated automated thrombogram.
Results: FVIII decreased during post-thaw storage; this was significant after 24 h for WB (p = 0.0002) and apheresis (p < 0.0001). All apheresis and eight of 20 WB cryoprecipitate met the FVIII specification (≥ 70 IU/unit) on day 14 post-thaw. Fibrinogen remained stable for 48 h, and components met the specification on day 14 post-thaw. There were no significant differences in VWF (WB p = 0.1292; apheresis p = 0.1507) throughout storage. There were small but significant decreases in thrombin generation lag time, endogenous thrombin potential and time to peak for both WB and apheresis cryoprecipitate.
Conclusion: Whilst coagulation factors in cryoprecipitate decreased after post-thaw storage, the thawed cryoprecipitate met the Council of Europe specifications when stored at refrigerated temperatures for 7 days.
(© 2024 Australian Red Cross Lifeblood. Vox Sanguinis published by John Wiley & Sons Ltd on behalf of International Society of Blood Transfusion.)
Databáze: MEDLINE
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