In vitro and in vivo evaluation of tedizolid nanoparticle incorporated buccal films for oromucosal infections.

Autor: Gültekin HE; Department of Pharmaceutical Technology, Faculty of Pharmacy, İzmir Kâtip Çelebi University, Çiğli, 35620 İzmir, Turkey. Electronic address: hazalezgi.gultekin@ikcu.edu.tr., Aydın HH; Department of Pharmaceutical Technology, Faculty of Pharmacy, İzmir Kâtip Çelebi University, Çiğli, 35620 İzmir, Turkey., Şahiner A; Department of Biology, Science Faculty, Ege University, 35100 İzmir, Turkey., Soylu FE; Laboratory Animals Research Center, Ege University, 35100 İzmir, Turkey., Şenyiğit Z; Department of Pharmaceutical Technology, Faculty of Pharmacy, İzmir Kâtip Çelebi University, Çiğli, 35620 İzmir, Turkey., Karayıldırım ÇK; Department of Biology, Science Faculty, Ege University, 35100 İzmir, Turkey; Laboratory Animals Research Center, Ege University, 35100 İzmir, Turkey.
Jazyk: angličtina
Zdroj: International journal of pharmaceutics [Int J Pharm] 2024 Nov 15; Vol. 665, pp. 124688. Date of Electronic Publication: 2024 Sep 16.
DOI: 10.1016/j.ijpharm.2024.124688
Abstrakt: A novel tedizolid phosphate (TZP) nanoparticle (NP)-loaded buccal film formulation was developed for the treatment of buccal wounds infected with S. aureus. TZP-loaded chitosan NPs were produced and characterized to prepare this composite system. The optimum NP formulation was then loaded into mucoadhesive buccal films. The antibacterial effects of the obtained buccal films were evaluated by in vitro and in vivo studies. The optimum TZP-NP formulation (F8) had a particle size of 177.40 ± 2.97 nm and PDI and ZP values were 0.437 ± 0.002 and 33.9 ± 0.5, respectively. In antibacterial efficacy tests, the optimum NP containing buccal film formulation was used, which released approximately 90 % of TZP within 5 h. TZP-NP-loaded buccal films achieved a 3 log 10 reduction in S. aureus within just 3 h. It was also administered to Wistar albino rats with S. aureus-infected buccal wounds. As a result of in vivo studies, a significant decrease in the number of S. aureus was detected in wound samples treated with TZP-NP-loaded buccal films. In addition, a complete inhibition of growth was observed on the fifth day of the film application. The current work suggested that the TZP-NP-loaded composite films could be promising candidates for effective and long-acting antibacterial treatment of buccal wounds.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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