Distinct transcriptional profiles in rat thyroid glands after developmental exposure to three in vitro thyroperoxidase inhibiting chemicals.

Autor: Rosenmai AK; National Food Institute, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark. Electronic address: akjro@food.dtu.dk., Svingen T; National Food Institute, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark., Evrard B; Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Santé, Environnement et Travail) - UMR_S 1085, F-35000 Rennes, France., Nguyen KH; National Food Institute, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark., Nielsen C; National Food Institute, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark., Axelstad M; National Food Institute, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark., Chalmel F; Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Santé, Environnement et Travail) - UMR_S 1085, F-35000 Rennes, France., Ramhøj L; National Food Institute, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
Jazyk: angličtina
Zdroj: Genomics [Genomics] 2024 Sep; Vol. 116 (5), pp. 110938. Date of Electronic Publication: 2024 Sep 16.
DOI: 10.1016/j.ygeno.2024.110938
Abstrakt: Thyroperoxidase (TPO) is central in thyroid hormone (TH) synthesis and inhibition can lead to TH deficiency. Many chemicals can inhibit TPO activity in vitro, but how this may manifest in the developing thyroid gland at the molecular level is unclear. Here, we characterized the thyroid gland transcriptome of male rats developmentally exposed to the in vitro TPO-inhibitors amitrole, 2-mercaptobenzimidazole (MBI), or cyanamide by use of Bulk-RNA-Barcoding (BRB) and sequencing. Amitrole exposure caused TH deficiency and 149 differentially expressed genes in the thyroid gland. The effects indicated an activated and growing thyroid gland. MBI caused intermittent changes to serum TH concentrations in a previous study and this was accompanied by 60 differentially expressed genes in the present study. More than half of these were also affected by amitrole, indicating that they could be early effect biomarkers of developmental TH system disruption due to TPO inhibition. Further work to validate the signature is needed, including assessment of substance independency and applicability domain.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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