A microfluidic paper-based fluorescent sensor integrated with a smartphone platform for rapid on-site detection of omethoate pesticide.

Autor: Liu S; College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China; Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment & Technology, School of Intelligent Manufacturing, Jiangnan University, Wuxi 214122, China., Zhao J; College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China., Wu J; College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China; Henan International Joint Laboratory of Laser Technology in Agriculture Sciences, Zhengzhou 450002, China., Wang L; College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China; Henan International Joint Laboratory of Laser Technology in Agriculture Sciences, Zhengzhou 450002, China., Yao C; College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China; Henan International Joint Laboratory of Laser Technology in Agriculture Sciences, Zhengzhou 450002, China., Hu J; College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China; Henan International Joint Laboratory of Laser Technology in Agriculture Sciences, Zhengzhou 450002, China; State Key Laboratory of Wheat and Maize Crop Science, Zhengzhou 450002, China., Zhang H; College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China; Henan International Joint Laboratory of Laser Technology in Agriculture Sciences, Zhengzhou 450002, China. Electronic address: hao.zhang@henau.edu.cn.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Sep 10; Vol. 463 (Pt 2), pp. 141205. Date of Electronic Publication: 2024 Sep 10.
DOI: 10.1016/j.foodchem.2024.141205
Abstrakt: A novel approach combing a fluorescent microfluidic paper strip with a portable smartphone-based sensing platform is developed for rapid and sensitive detection of omethoate pesticide. The detection mechanism of the microfluidic paper strip is based on the fluorescence quenching of graphene oxide (GO) toward the cyanine 3 (Cy3)-labeled aptamer (Cy3-APT). Upon exposure to omethoate, the Cy3-APT detaches from the surface of GO, resulting in considerable fluorescence recovery, which can be visualized through the smartphone-based sensing platform. The images are analyzed through a self-developed app embedded with a pretrained convolutional neural network model, achieving a high regression coefficient of 0.9964 at an omethoate concentration range of 0-750 nM. The smartphone-based platform enables rapid on-site detection of omethoate pesticide in real samples within 10 min, with results comparable to those obtained using standard methods. In short, the proposed microfluidic paper-based fluorescent sensor combined with the smartphone-based sensing platform enhances the detection performance toward organophosphorus pesticides.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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