Development of genogroup-specific ELISAs based on the VP1 protein to detect antibodies to GIV and GVI feline norovirus.

Autor: Takano T; School of Veterinary Medicine, Kitasato University, Towada, Aomori, 034-8628, Japan. takanot@vmas.kitasato-u.ac.jp., Tsuhama H; School of Veterinary Medicine, Kitasato University, Towada, Aomori, 034-8628, Japan., Matsumoto Y; School of Veterinary Medicine, Kitasato University, Towada, Aomori, 034-8628, Japan., Doki T; School of Veterinary Medicine, Kitasato University, Towada, Aomori, 034-8628, Japan., Kusuhara H; Health and Environment Research Institute, Yokkaichi, Mie, 512-1211, Japan.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2024 Sep 18; Vol. 169 (10), pp. 201. Date of Electronic Publication: 2024 Sep 18.
DOI: 10.1007/s00705-024-06137-w
Abstrakt: Feline norovirus (FNoV) is a potential pathogen of feline gastroenteritis and has two genogroups (GIV and GVI). Few epidemiological studies have been conducted on FNoV. We designed two enzyme-linked immunosorbent assays (ELISAs) to identify genogroup-specific FNoV antibodies for serological surveillance. Analysis of sera from cats experimentally infected with FNoV GIV or GVI and from specific-pathogen-free (SPF) cats confirmed that the two recombinant proteins used in the assay react in a genogroup-specific manner. Of the 183 samples tested, 6.6% were positive for GIV and 26.2% were positive for GVI. Antibodies to both FNoV genogroups were detected in sera collected in 2005, seven years before FNoV was first reported.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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