Intracellular lipid droplets are exploited by Junín virus in a nucleoprotein-dependent process.

Autor:	Vazquez CA; Laboratorio de Procesos Moleculares de la Interacción Virus-Célula, Departamento de Química Biológica (QB), Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA)-Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, C1428EHA, Argentina.; Laboratorio de Estrategias Antivirales, Departamento de Química Biológica (QB), Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA)-Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, C1428EHA, Argentina., Escudero-Pérez B; Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.; German Center for Infection Research (DZIF), Partner Site Hamburg-Lübeck-Borstel-Riems, 20359 Hamburg, Germany., Hayashi JM; J. David Gladstone Institutes, San Francisco, CA 94158, USA., Leon KE; J. David Gladstone Institutes, San Francisco, CA 94158, USA., Moreira JP; J. David Gladstone Institutes, San Francisco, CA 94158, USA., Castañeda Cataña MA; Laboratorio de Estrategias Antivirales, Departamento de Química Biológica (QB), Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA)-Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, C1428EHA, Argentina., Groseth A; Laboratory for Arenavirus Biology, Institute of Molecular Virology and Cell Biology Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany., Ott M; J. David Gladstone Institutes, San Francisco, CA 94158, USA., Oestereich L; Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.; German Center for Infection Research (DZIF), Partner Site Hamburg-Lübeck-Borstel-Riems, 20359 Hamburg, Germany., Muñoz-Fontela C; Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.; German Center for Infection Research (DZIF), Partner Site Hamburg-Lübeck-Borstel-Riems, 20359 Hamburg, Germany., Garcia CC; Laboratorio de Estrategias Antivirales, Departamento de Química Biológica (QB), Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA)-Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, C1428EHA, Argentina., Cordo SM; Laboratorio de Procesos Moleculares de la Interacción Virus-Célula, Departamento de Química Biológica (QB), Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA)-Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, C1428EHA, Argentina.
Jazyk:	angličtina
Zdroj:	Journal of cell science [J Cell Sci] 2024 Oct 01; Vol. 137 (19). Date of Electronic Publication: 2024 Oct 14.
DOI:	10.1242/jcs.261745
Abstrakt:	Lipid droplets (LDs) are organelles involved in lipid storage, maintenance of energy homeostasis, protein sequestration, signaling events and inter-organelle interactions. Recently, LDs have been shown to favor the replication of members from different viral families, such as the Flaviviridae and Coronaviridae. In this work, we show that LDs are essential organelles for members of the Arenaviridae family. A virus-driven reduction of LD number was observed in cultures infected with Junín mammarenavirus (JUNV), caused in part by action of the viral nucleoprotein. Notably, we identified a new pool of nucleoprotein and viral RNA that localizes in the vicinity of LDs, suggesting that LDs play a role during the viral replication cycle. Regarding the mechanism behind LD exhaustion, we found evidence that lipophagy is involved in LD degradation with the resulting fatty acids being substrates of fatty acid β-oxidation, which fuels viral multiplication. This work highlights the importance of LDs during the replication cycle of JUNV, contributing to the knowledge of the metabolic changes these mammarenaviruses cause in their hosts. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2024. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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