Anastomotic leak occurs independently from microbiota shifts associated with surgical bowel preparation.

Autor: Boatman S; Basic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA., Khan MH; Basic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA., Ganesan N; Basic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA., Nalluri-Butz H; Basic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA., Kohn J; Basic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA., Troester A; Basic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA., Ziegert Z; Basic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA., Madoff R; Basic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA.; Division of Colon and Rectal Surgery, University of Minnesota, 420 Delaware Street SE, MMC 450, Minneapolis, MN, 55455, USA., Gaertner WB; Basic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA.; Division of Colon and Rectal Surgery, University of Minnesota, 420 Delaware Street SE, MMC 450, Minneapolis, MN, 55455, USA., Jahansouz C; Basic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA. jahan023@umn.edu.; Division of Colon and Rectal Surgery, University of Minnesota, 420 Delaware Street SE, MMC 450, Minneapolis, MN, 55455, USA. jahan023@umn.edu., Staley C; Basic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA. cmstaley@umn.edu.; BioTechnology Institute, University of Minnesota, St. Paul, MN, 55108, USA. cmstaley@umn.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Sep 17; Vol. 14 (1), pp. 21711. Date of Electronic Publication: 2024 Sep 17.
DOI: 10.1038/s41598-024-72520-x
Abstrakt: Following bowel surgery, infectious complications, including anastomotic leak (AL), remain major sources of morbidity and mortality. Bowel preparation is often administered with the assumption that gut decontamination reduces post-surgical complications. In this study, we tested this hypothesis using a murine model of colon surgery. The mice were fed either regular chow or a high-fat, high-sugar Western diet. The day before surgery, the mice received one of four interventions: water (control), mechanical bowel preparation (MBP), oral antibiotics (OA), or both MBP and OA. We found no differences in the rates of AL among the experimental groups, and diet did not appear to affect the outcomes. Exploratory analyses showed changes in the gut microbiome consistent with the different treatments, but investigations of fecal short-chain fatty acids and RNA sequencing of colonic tissue did not reveal specific effects of the treatments or the presence of AL. However, we did identify bacterial genera that may be causally associated with AL and developed a predictive index from stool samples as a marker for the presence of AL. Future research is needed to identify and validate a microbial predictive tool and to uncover the microbial-driven mechanisms that lead to AL.
(© 2024. The Author(s).)
Databáze: MEDLINE
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