Harnessing noncanonical redox cofactors to advance synthetic assimilation of one-carbon feedstocks.

Autor: Orsi E; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark. Electronic address: enricoo@biosustain.dtu.dk., Hernández-Sancho JM; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark., Remeijer MS; Amsterdam Institute for Life and Environment and Institute of Molecular and Life Sciences, Vrije Universiteit, Amsterdam, the Netherlands., Kruis AJ; Acies Bio D.O.O., Ljubljana, Slovenia., Volke DC; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark., Claassens NJ; Microbiology, Wageningen University and Research, Wageningen, the Netherlands., Paul CE; Department of Biotechnology, Delft University of Technology, the Netherlands., Bruggeman FJ; Amsterdam Institute for Life and Environment and Institute of Molecular and Life Sciences, Vrije Universiteit, Amsterdam, the Netherlands., Weusthuis RA; Bioprocess Engineering, Wageningen University and Research, Wageningen, the Netherlands., Nikel PI; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark. Electronic address: pabnik@biosustain.dtu.dk.
Jazyk: angličtina
Zdroj: Current opinion in biotechnology [Curr Opin Biotechnol] 2024 Dec; Vol. 90, pp. 103195. Date of Electronic Publication: 2024 Sep 16.
DOI: 10.1016/j.copbio.2024.103195
Abstrakt: One-carbon (C1) feedstocks, such as carbon monoxide (CO), formate (HCO 2 H), methanol (CH 3 OH), and methane (CH 4 ), can be obtained either through stepwise electrochemical reduction of CO 2 with renewable electricity or via processing of organic side streams. These C1 substrates are increasingly investigated in biotechnology as they can contribute to a circular carbon economy. In recent years, noncanonical redox cofactors (NCRCs) emerged as a tool to generate synthetic electron circuits in cell factories to maximize electron transfer within a pathway of interest. Here, we argue that expanding the use of NCRCs in the context of C1-driven bioprocesses will boost product yields and facilitate challenging redox transactions that are typically out of the scope of natural cofactors due to inherent thermodynamic constraints.
Competing Interests: Declaration of Competing Interest Nothing declared.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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