| Autor: |
Zheng Y; Institute for Oilseed Crop Germplasm Innovation and Utilization, Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China., Guo T; Institute for Oilseed Crop Germplasm Innovation and Utilization, Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China., Xia T; Institute for Oilseed Crop Germplasm Innovation and Utilization, Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China., Guo S; Institute for Oilseed Crop Germplasm Innovation and Utilization, Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China., Chen M; Institute for Oilseed Crop Germplasm Innovation and Utilization, Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China., Ye S; Institute for Oilseed Crop Germplasm Innovation and Utilization, Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China., Pan T; Institute for Oilseed Crop Germplasm Innovation and Utilization, Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China., Xu X; Institute for Oilseed Crop Germplasm Innovation and Utilization, Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China., Gan Y; Institute for Oilseed Crop Germplasm Innovation and Utilization, Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China., Zhan Y; Institute for Oilseed Crop Germplasm Innovation and Utilization, Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China., Zheng T; College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.; Zhejiang University Zhongyuan Institute, Zhengzhou 450000, China., Zheng Z; Institute for Oilseed Crop Germplasm Innovation and Utilization, Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China. |
| Abstrakt: |
This study reports the use of the Arabidopsis KASII promoter ( AtKASII ) to develop an efficient CRISPR/Cas9 system for soybean genome editing. When this promoter was paired with Arabidopsis U6 promoters to drive Cas9 and single guide RNA expression, respectively, simultaneous editing of the three fatty acid desaturase genes GmFAD2-1A , GmFAD2-1B , and GmFAD3A occurred in more than 60% of transgenic soybean lines at T 2 generation, and all the triple mutants possessed desirable high-oleic traits. In sharp contrast, not a single line underwent simultaneous editing of the three target genes when AtKASII was replaced by the widely used AtEC1.2 promoter. Furthermore, our study showed that the stable and inheritable mutations in the high-oleic lines did not alter the overall contents of oil and protein or amino acid composition while increasing the oleic acid content up to 87.6% from approximately 23.8% for wild-type seeds, concomitant with 34.4- and 3.7-fold reductions in linoleic and linolenic acid, respectively. Collectively, this study demonstrates that the AtKASII promoter is highly promising for optimization of the CRISPR/Cas9 system for genome editing in soybean and possibly beyond. |
