Antioxidant Activities of Exopolysaccharides Extracts from Two Endemic Fungi from Patagonia.

Autor: Gallo AL; Centro de Investigación y Extensión Forestal Andino Patagónico (CIEFAP), ruta 259 km 16, CP 9200, Esquel, Chubut, Argentina.; Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+I), Buenos Aires, Argentina.; Universidad Nacional de la Patagonia San Juan Bosco (UNPSJB), ruta 259, CP 9200, Esquel, Chubut, Argentina., Marfetán JA; Centro de Investigación y Extensión Forestal Andino Patagónico (CIEFAP), ruta 259 km 16, CP 9200, Esquel, Chubut, Argentina.; CONICET Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina., Vélez ML; Centro de Investigación y Extensión Forestal Andino Patagónico (CIEFAP), ruta 259 km 16, CP 9200, Esquel, Chubut, Argentina. mvelez@ciefap.org.ar.; Universidad Nacional de la Patagonia San Juan Bosco (UNPSJB), ruta 259, CP 9200, Esquel, Chubut, Argentina. mvelez@ciefap.org.ar.; CONICET Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina. mvelez@ciefap.org.ar.
Jazyk: angličtina
Zdroj: Current microbiology [Curr Microbiol] 2024 Sep 17; Vol. 81 (11), pp. 361. Date of Electronic Publication: 2024 Sep 17.
DOI: 10.1007/s00284-024-03883-7
Abstrakt: A great number of free radicals have a negative impact on the human body, and an increased interest in the identification of new natural molecules with antioxidant properties has emerged due to concerns about synthetic antioxidants. Here, the antioxidant effect of four exo-polysaccharides (EPS) extracts obtained from submerged cultivation of Nothophellinus andinopatagonicus and Pseudoinonotus crustosus (N and P, respectively) in two culture media (M1 and M2) at 2 concentrations (100 and 250 µg/ml) was studied; then, its relation with the chemical composition of the EPS was evaluated. To assess the antioxidant activities of the extracts, several in vitro assays were performed: DPPH and ABTS radical scavenging, ferric-reducing antioxidant power, chelating ability on ferrous ions, and inhibition of the lipid peroxidation. The concentrations tested here were much lower than those reported in previous works. Despite variations in chemical composition and monosaccharide profiles among the extracts, all demonstrated antioxidant activity, although the type of activity differed; only P-M1 exhibited a good antioxidant activity across all assays. This extract contained the highest proportion of phenolic compounds, and also displayed the highest radical scavenging activity. Although the utilization of polysaccharides as functional food ingredients remains limited, we propose P-M1 as a promising candidate for a nutraceutical product. Additionally, a formulation could be made with a combination of extracts to create an antioxidant-rich supplement. Additional research is needed to confirm our findings in a cellular environment and to elucidate the mechanisms that drive their antioxidant activities, ultimately facilitating their development and utilization as nutraceutical products.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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