Hepato-renal protective impact of nanocapsulated Petroselinum crispum and Anethum graveolens essential oils added in fermented milk against some food additives via antioxidant and anti-inflammatory effects: In silico and in vivo studies.

Autor:	Mohamed RS; Nutrition and Food Sciences Department, National Research Centre, Dokki, Cairo, Egypt., Fouda K; Nutrition and Food Sciences Department, National Research Centre, Dokki, Cairo, Egypt., Maghraby AS; Department of Therapeutic Chemistry, research group immune-and bio-markers for infection, the Center of Excellent for Advanced Science (CEAS), National Research Centre, Dokki, Cairo, Egypt., Assem FM; Dairy Science Department, National Research Centre, Dokki, Cairo, Egypt., Menshawy MM; College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology, 6th October City, Egypt., Zaghloul AH; Dairy Science Department, National Research Centre, Dokki, Cairo, Egypt., Abdel-Salam AM; Dairy Science Department, National Research Centre, Dokki, Cairo, Egypt.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2024 Aug 30; Vol. 10 (17), pp. e36866. Date of Electronic Publication: 2024 Aug 30 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2024.e36866
Abstrakt:	The study assessed the efficacy of parsley and dill essential oils (EOs) nanocapsules incorporated into fermented milk in hepato-renal protection against specific food additives. A molecular docking assay was conducted between parsley and dill EOs bioactive molecules and inflammatory cytokines. Freeze-dried parsley and dill EOs nanocapsules were developed, characterized for their morphological structure, particle size, zeta potential, polydispersity index and encapsulation efficiency and assessed in fast green dye and sodium benzoate (SB) combination-treated rats. The docking results revealed that the primary constituents of parsley and dill EOs (apiol, myristicin, α-pinene, (-)-carvone, and d-limonene) interacted with the active sites of TNF-α, IL-1β and TGF-1β cytokines with hydrophobic and hydrogen bond interactions. D-limonene had the highest binding affinity (6.4 kcal/mol) for the TNF-α. Apiol and myristicin had the highest binding affinity (5.1, 5.0, 5.0 and 5.0 kcal/mol, respectively) for the IL-1β and TGF-β1 receptors. Biochemically and histopathologically, the excessive co-administration of fast green and SB revealed adverse effects on the liver and the kidney. Whereas the treatment with parsley and dill EOs nanocapsules afford hepato-renal protective effects as manifested by suppression the elevated liver and kidney functions. Parsley and dill EOs nanocapsules showed a significant reduction of the liver (64.08 and 80.5 pg/g, respectively) and kidney (59.3 and 83.6 pg/g, respectively) ROS. Moreover, parsley and dill EOs nanocapsules down-regulated the liver and the kidney inflammatory cytokines (IL-6, TNF-α, IL-1β and TGF-1β) and lipid peroxidation and up-regulated the antioxidant enzymes. In conclusion, the data suggest a potential hepato-renal protective effects of parsley and dill EOs nanocapsules. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Author(s).)
Databáze:	MEDLINE
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