Aqueous extraction of formalin-fixed paraffin-embedded tissue and detection of prion disease using real-time quaking-induced conversion.

Autor: Nicholson EM; Virus and Prion Research Unit, National Animal Disease Center, USDA-ARS, 1920 Dayton Ave., Ames, IA, 50010, USA. Eric.Nicholson@usda.gov., Greenlee JJ; Virus and Prion Research Unit, National Animal Disease Center, USDA-ARS, 1920 Dayton Ave., Ames, IA, 50010, USA., Hwang S; Virus and Prion Research Unit, National Animal Disease Center, USDA-ARS, 1920 Dayton Ave., Ames, IA, 50010, USA.; Center for Veterinary Biologics, USDA-APHIS, 1920 Dayton Avenue, Ames, IA, 50010, USA.
Jazyk: angličtina
Zdroj: BMC research notes [BMC Res Notes] 2024 Sep 16; Vol. 17 (1), pp. 266. Date of Electronic Publication: 2024 Sep 16.
DOI: 10.1186/s13104-024-06886-6
Abstrakt: Objective: The goal of the research presented here is to determine if methods previously developed for the aqueous extraction of PrP Sc from formalin-fixed paraffin-embedded tissue (FFPET) are applicable to the detection PrP Sc by real-time quaking induced conversion (RT-QuIC). Previous work has utilized aqueous extraction of FFPET for detection of transmissible spongiform encephalopathies (TSEs) utilizing western blot and ELISA. This research extends the range of suitable methods for detection of TSEs in FFPET to RT-QuIC, which is arguably the most sensitive method to detect TSEs.
Results: We found complete agreement between the TSE status and the results from RT-QuIC seeded with the aqueous extract of FFPET samples. The method affords the diagnostic assessment TSE status by RT-QuIC of FFPET without the use of organic solvents that would otherwise create a mixed chemical-biological waste for disposal.
(© 2024. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply [YEAR] .)
Databáze: MEDLINE
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