Dynamics and necessity of SIRT1 for maternal-zygotic transition.

Autor: Nevoral J; Faculty of Medicine in Pilsen, Biomedical Center, Charles University, Alej Svobody 76, 323 00, Pilsen, Czech Republic. jan.nevoral@lfp.cuni.cz.; Faculty of Medicine in Pilsen, Department of Histology and Embryology, Charles University, Alej Svobody 76, 323 00, Pilsen, Czech Republic. jan.nevoral@lfp.cuni.cz., Drutovic D; Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, Rumburska 89, 277 21, Libechov, Czech Republic., Vaskovicova M; Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, Rumburska 89, 277 21, Libechov, Czech Republic., Benc M; Faculty of Natural Sciences and Informatics, Constantine the Philosopher University in Nitra, Nabrezie Mladeze 91, 94974, Nitra, Slovakia., Liska F; First Faculty of Medicine, Institute of Biology and Medical Genetics, Charles University, Kateřinská 1660/32, 121 08, Prague, Czech Republic., Valentova I; Faculty of Medicine in Pilsen, Biomedical Center, Charles University, Alej Svobody 76, 323 00, Pilsen, Czech Republic.; Pronatal Sanatorium, Na Dlouhé Mezi 12/4, 147 00, Prague 4, Czech Republic., Stachovicova S; Faculty of Natural Sciences and Informatics, Constantine the Philosopher University in Nitra, Nabrezie Mladeze 91, 94974, Nitra, Slovakia.; Université Paris-Saclay, Université de Versailles Saint-Quentin-en-Yvelines, INRAE, BREED, Jouy-en-Josas, France., Kubovciak J; Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics of the Czech Academy of Sciences, Vídeňská, 1083, 142 20, Prague 4, Czech Republic., Havrankova J; Faculty of Medicine in Pilsen, Biomedical Center, Charles University, Alej Svobody 76, 323 00, Pilsen, Czech Republic.; Faculty of Medicine in Pilsen, Department of Histology and Embryology, Charles University, Alej Svobody 76, 323 00, Pilsen, Czech Republic., Shavit M; Faculty of Medicine in Pilsen, Biomedical Center, Charles University, Alej Svobody 76, 323 00, Pilsen, Czech Republic., Monsef L; Faculty of Medicine in Pilsen, Biomedical Center, Charles University, Alej Svobody 76, 323 00, Pilsen, Czech Republic., Iniesta-Cuerda M; Faculty of Medicine in Pilsen, Biomedical Center, Charles University, Alej Svobody 76, 323 00, Pilsen, Czech Republic., Zalmanova T; Faculty of Medicine in Pilsen, Biomedical Center, Charles University, Alej Svobody 76, 323 00, Pilsen, Czech Republic.; Institute of Animal Science, Přátelství 815, Uhříněves, 104 00, Prague, Czech Republic., Hosek P; Faculty of Medicine in Pilsen, Biomedical Center, Charles University, Alej Svobody 76, 323 00, Pilsen, Czech Republic., Strejcek F; Faculty of Natural Sciences and Informatics, Constantine the Philosopher University in Nitra, Nabrezie Mladeze 91, 94974, Nitra, Slovakia., Kralickova M; Faculty of Medicine in Pilsen, Biomedical Center, Charles University, Alej Svobody 76, 323 00, Pilsen, Czech Republic.; Faculty of Medicine in Pilsen, Department of Histology and Embryology, Charles University, Alej Svobody 76, 323 00, Pilsen, Czech Republic., Petr J; Institute of Animal Science, Přátelství 815, Uhříněves, 104 00, Prague, Czech Republic.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Sep 16; Vol. 14 (1), pp. 21598. Date of Electronic Publication: 2024 Sep 16.
DOI: 10.1038/s41598-024-72595-6
Abstrakt: Dynamic changes in maternal‒zygotic transition (MZT) require complex regulation of zygote formation, maternal transcript decay, embryonic genome activation (EGA), and cell cycle progression. Although these changes are well described, some key regulatory factors are still elusive. Sirtuin-1 (SIRT1), an NAD + -dependent histone deacetylase, is a versatile driver of MZT via its epigenetic and nonepigenetic substrates. This study focused on the dynamics of SIRT1 in early embryos and its contribution to MZT. A conditional SIRT1-deficient knockout mouse model was used, accompanied by porcine and human embryos. Embryos across mammalian species showed the prominent localization of SIRT1 in the nucleus throughout early embryonic development. Accordingly, SIRT1 interacts with histone H4 on lysine K16 (H4K16) in both mouse and human blastocysts. While maternal SIRT1 is dispensable for MZT, at least one allele of embryonic Sirt1 is required for early embryonic development around the time of EGA. This role of SIRT1 is surprisingly mediated via a transcription-independent mode of action.
(© 2024. The Author(s).)
Databáze: MEDLINE
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