Contribution of major histocompatibility complex class II immunostaining in distinguishing idiopathic inflammatory myopathy subgroups: A histopathological cohort study.

Autor:	Lessard LER; Service d'Electroneuromyographie et de pathologies neuromusculaires, Hôpital Neurologique, GHE, Hospices Civils de Lyon, Lyon, France.; Institut NeuroMyoGène, Unité Physiopathologie et Génétique du Neurone et du Muscle, CNRS UMR 5261, Inserm U1315, Université Claude Bernard Lyon 1, Lyon, France., Robert M; Service de Médecine interne et immunologie clinique, Centre Hospitalier Universitaire Édouard Herriot, Hospices Civils de Lyon, Lyon, France., Fenouil T; Institut de Pathologie Multisite des Hospices Civils de Lyon-Site Est, GHE, Hospices Civils de Lyon, Lyon, France.; Centre de Recherche en Cancérologie de Lyon, Inserm U1052, CNRS UMR 5286, Lyon, France., Mounier R; Institut NeuroMyoGène, Unité Physiopathologie et Génétique du Neurone et du Muscle, CNRS UMR 5261, Inserm U1315, Université Claude Bernard Lyon 1, Lyon, France., Landel V; Direction de la Recherche en Santé, Hospices Civils de Lyon, Lyon, France., Carlesimo M; Institut de Pathologie Multisite des Hospices Civils de Lyon-Site Est, GHE, Hospices Civils de Lyon, Lyon, France., Hot A; Service de Médecine interne et immunologie clinique, Centre Hospitalier Universitaire Édouard Herriot, Hospices Civils de Lyon, Lyon, France., Chazaud B; Institut NeuroMyoGène, Unité Physiopathologie et Génétique du Neurone et du Muscle, CNRS UMR 5261, Inserm U1315, Université Claude Bernard Lyon 1, Lyon, France., Laumonier T; Laboratoire 'Cell Therapy & Musculoskeletal Disorders', Département de Chirurgie Orthopédique, Hôpital Universitaire et Faculté de Médecine, Genève, Switzerland., Streichenberger N; Institut NeuroMyoGène, Unité Physiopathologie et Génétique du Neurone et du Muscle, CNRS UMR 5261, Inserm U1315, Université Claude Bernard Lyon 1, Lyon, France.; Institut de Pathologie Multisite des Hospices Civils de Lyon-Site Est, GHE, Hospices Civils de Lyon, Lyon, France., Gallay L; Service de Médecine interne et immunologie clinique, Centre Hospitalier Universitaire Édouard Herriot, Hospices Civils de Lyon, Lyon, France.; Laboratoire 'Cell Therapy & Musculoskeletal Disorders', Département de Chirurgie Orthopédique, Hôpital Universitaire et Faculté de Médecine, Genève, Switzerland.
Jazyk:	angličtina
Zdroj:	Journal of neuropathology and experimental neurology [J Neuropathol Exp Neurol] 2024 Sep 16. Date of Electronic Publication: 2024 Sep 16.
DOI:	10.1093/jnen/nlae098
Abstrakt:	Idiopathic inflammatory myopathies (IIM) are rare, acquired muscle diseases; their diagnosis of is based on clinical, serological, and histological criteria. MHC-I-positive immunostaining, although non-specific, is used as a marker for IIM diagnosis; however, the significance of major histocompatibility complex (MHC)-II immunostaining in IIM remains debated. We investigated patterns of MHC-II immunostaining in myofibers and capillaries in muscle biopsies from 103 patients with dermatomyositis ([DM], n = 31), inclusion body myositis ([IBM], n = 24), anti-synthetase syndrome ([ASyS], n = 10), immune-mediated necrotizing myopathy ([IMNM], n = 18), or overlap myositis ([OM], n = 20). MHC-II immunostaining of myofibers was abnormal in 63/103 of patients (61%) but the patterns differed according to the IIM subgroup. They were diffuse in IBM (96%), negative in IMNM (83%), perifascicular in ASyS (70%), negative (61%) or perifascicular (32%) in DM, and either clustered (40%), perifascicular (30%), or diffuse heterogeneous (15%) in OM. Capillary MHC-II immunostaining also identified quantitative (capillary dropout, n = 47/88, 53%) and qualitative abnormalities, that is, architectural abnormalities, including dilated and leaky capillaries, (n = 79/98, 81%) in all IIM subgroups. Thus, MHC-II myofiber expression patterns allow distinguishing among IIM subgroups. We suggest the addition of MHC-II immunostaining to routine histological panels for IIM diagnosis. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Association of Neuropathologists, Inc.)
Databáze:	MEDLINE
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