Harnessing glycofluoroforms for impedimetric biosensing.

Autor:	Hewson AR; Department of Chemistry, University of York YO10 5DD York UK alison.parkin@york.ac.uk martin.fascione@york.ac.uk., Lloyd-Laney HO; Department of Chemistry, University of York YO10 5DD York UK alison.parkin@york.ac.uk martin.fascione@york.ac.uk., Keenan T; Department of Chemistry, University of York YO10 5DD York UK alison.parkin@york.ac.uk martin.fascione@york.ac.uk., Richards SJ; Department of Chemistry, The University of Manchester M13 9PL UK.; Manchester Institute of Biotechnology, The University of Manchester M1 7DN UK., Gibson MI; Department of Chemistry, The University of Manchester M13 9PL UK.; Manchester Institute of Biotechnology, The University of Manchester M1 7DN UK., Linclau B; Department of Organic and Macromolecular Chemistry, Ghent University Krijgslaan 281-S4 9000 Gent Belgium.; School of Chemistry, University of Southampton Highfield Southampton SO17 1BJ UK., Signoret N; Hull York Medical School, University of York YO10 5DD York UK., Fascione MA; Department of Chemistry, University of York YO10 5DD York UK alison.parkin@york.ac.uk martin.fascione@york.ac.uk., Parkin A; Department of Chemistry, University of York YO10 5DD York UK alison.parkin@york.ac.uk martin.fascione@york.ac.uk.
Jazyk:	angličtina
Zdroj:	Chemical science [Chem Sci] 2024 Sep 13. Date of Electronic Publication: 2024 Sep 13.
DOI:	10.1039/d4sc04409f
Abstrakt:	Glycans play a major role in biological cell-cell recognition and signal transduction but have found limited application in biosensors due to glycan/lectin promiscuity; multiple proteins are capable of binding to the same native glycan. Here, site-specific fluorination is used to introduce protein-glycan selectivity, and this is coupled with an electrochemical detection method to generate a novel biosensor platform. 3F-lacto- N -biose glycofluoroform is installed onto polymer tethers, which are subsequently immobilised onto gold screen printed electrodes, providing a non-fouling surface. The impedance biosensing platform is shown to selectively bind cancer-associated galectin-3 compared to control glycans and proteins. To improve the analytical capability, Bayesian statistical analysis was deployed in the equivalent circuit fitting of electrochemical impedance spectroscopy data. It is shown that Markov Chain Monte Carlo (MCMC) analysis is a helpful method for visualising experimental irreproducibility, and we apply this as a quality control step. Competing Interests: The authors declare no competing financial interest. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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