Evaluating Mechanisms of Soil Microbiome Suppression of Striga Infection in Sorghum.

Autor:	Taylor T; Department of Plant Biology and Genome Center, University of California, Davis, CA, USA.; Plant Biology Graduate Group, University of California, Davis, CA, USA., Daksa J; Department of Plant Biology and Genome Center, University of California, Davis, CA, USA., Shimels MZ; Netherlands Institute of Ecology (NIOO-KNAW), Department of Microbial Ecology, Wageningen, The Netherlands., Etalo DW; Netherlands Institute of Ecology (NIOO-KNAW), Department of Microbial Ecology, Wageningen, The Netherlands., Thiombiano B; Plant Hormone Biology Group, Green Life Sciences Cluster, University of Amsterdam, Amsterdam, The Netherlands., Walmsey A; Plant Hormone Biology Group, Green Life Sciences Cluster, University of Amsterdam, Amsterdam, The Netherlands., Chen AJ; Department of Plant Biology and Genome Center, University of California, Davis, CA, USA., Bouwmeester HJ; Plant Hormone Biology Group, Green Life Sciences Cluster, University of Amsterdam, Amsterdam, The Netherlands., Raaijmakers JM; Netherlands Institute of Ecology (NIOO-KNAW), Department of Microbial Ecology, Wageningen, The Netherlands., Brady SM; Department of Plant Biology and Genome Center, University of California, Davis, CA, USA., Kawa D; Department of Plant Biology and Genome Center, University of California, Davis, CA, USA.; Experimental and Computational Plant Development, Utrecht University, The Netherlands.; Plant Stress Resilience, Utrecht University, The Netherlands.
Jazyk:	angličtina
Zdroj:	Bio-protocol [Bio Protoc] 2024 Sep 05; Vol. 14 (17), pp. e5058. Date of Electronic Publication: 2024 Sep 05 (Print Publication: 2024).
DOI:	10.21769/BioProtoc.5058
Abstrakt:	The root parasitic weed Striga hermonthica has a devastating effect on sorghum and other cereal crops in Sub-Saharan Africa. Available Striga management strategies are rarely sufficient or not widely accessible or affordable. Identification of soil- or plant-associated microorganisms that interfere in the Striga infection cycle holds potential for development of complementary biological control measures. Such inoculants should be preferably based on microbes native to the regions of their application. We developed a method to assess microbiome-based soil suppressiveness to Striga with a minimal amount of field-collected soil. We previously used this method to identify the mechanisms of microbe-mediated suppression of Striga infection and to test individual microbial strains. Here, we present protocols to assess the functional potential of the soil microbiome and individual bacterial taxa that adversely affect Striga parasitism in sorghum via three major known suppression mechanisms. These methods can be further extended to other Striga hosts and other root parasitic weeds. Key features • This protocol provides a detailed description of the methods used in Kawa et al. [1]. • This protocol is optimized to assess soil suppressiveness to Striga infection by using natural field-collected soil and the same soil sterilized by gamma-radiation. • This protocol is optimized to test bacterial (and not fungal) isolates. • This protocol can be easily extended to other host-parasite-microbiome systems. Competing Interests: Competing interestsThere are no conflicts of interest or competing interests. (©Copyright : © 2024 The Authors; This is an open access article under the CC BY license.)
Databáze:	MEDLINE
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