Presence and mRNA Expression of the sar Family Genes in Clinical and Non-clinical (Healthy Conjunctiva and Healthy Skin) Isolates of Staphylococcus epidermidis .

Autor: Cancino-Diaz ME; Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11350 Ciudad de Mexico, Mexico., Gómez-Chávez F; Laboratorio de Enfermedades Osteoarticulares E Inmunológicas, Sección de Estudios de Posgrado E Investigación, ENMyH-Instituto Politécnico Nacional, 07320 Ciudad de Mexico, Mexico., Cancino-Diaz JC; Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11350 Ciudad de Mexico, Mexico.
Jazyk: angličtina
Zdroj: Indian journal of microbiology [Indian J Microbiol] 2024 Sep; Vol. 64 (3), pp. 1301-1309. Date of Electronic Publication: 2024 Jul 02.
DOI: 10.1007/s12088-024-01339-x
Abstrakt: Staphylococcus aureus possesses sar family genes, including sar A, S, R, T, U, V, X, Y, Z, and rot , which are transcription factors involved in biofilm formation and quorum sensing. In contrast, Staphylococcus epidermidis has sar A, R, V, X, Y, Z, and rot genes; specifically, SarA, Z, and X are involved in biofilm formation. The expression of the sar family members in S. epidermidis isolated from clinical and non-clinical environments is unknown. This study aimed to establish if clinical and non-clinical isolates of S. epidermidis express the sar family members. We genotyped isolates from clinical ocular infections (n = 52), or non-clinical healthy conjunctiva (n = 40), and healthy skin (n = 50), using multilocus sequence typing (MLST) and the staphylococcal chromosomal cassette mec (SCC mec ). We selected strains with different genotypes and representatives of each source of isolation, and the presence of the sar family genes was detected using PCR and RT-qPCR to determine their expression. The sar family genes were present in all selected strains, with no observed differences. The relative expression of the sar family showed that all selected strains expressed each gene weakly, with no significant differences observed between them or between different sources of isolation. In conclusion, the presence and relative expression of the sar family genes are very similar among strains, with no differences based on their origin of isolation and genotype.
Supplementary Information: The online version contains supplementary material available at 10.1007/s12088-024-01339-x.
Competing Interests: Conflict of interestAll authors declare that there is no commercial interest in this study.
(© Association of Microbiologists of India 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
Databáze: MEDLINE
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