Turbolysis: A low-cost, small footprint alternative to commercial bead beaters for cell lysis.
| Autor: | Limberis JD; Division of Pulmonary and Critical Care Medicine, Zuckerberg San Francisco General Hospital and Trauma Centre, University of California, San Francisco, San Francisco, CA, USA., Metcalfe JZ; Division of Pulmonary and Critical Care Medicine, Zuckerberg San Francisco General Hospital and Trauma Centre, University of California, San Francisco, San Francisco, CA, USA. |
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| Jazyk: | angličtina |
| Zdroj: | HardwareX [HardwareX] 2024 Aug 27; Vol. 19, pp. e00576. Date of Electronic Publication: 2024 Aug 27 (Print Publication: 2024). |
| DOI: | 10.1016/j.ohx.2024.e00576 |
| Abstrakt: | turboLysis is a novel mechanical cell lysis device that utilizes small beads to efficiently lyse tough cells like Mycobacterium, Saccharomyces, and Arabidopsis. We compared turboLysis to bead beating using the BeadBug 6 for several concentrations of Mycobacterium tuberculosis roughly correlated to the bacterial load commonly seen in patient samples. turboLysis performed similarly to the BeadBug at low bacterial concentrations and outperformed it at high concentrations above 2x10 5 CFU/ml (p < 0.005). Thus, turboLysis offers good cell lytic performance in a small form factor at a low cost. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Author(s).) |
| Databáze: | MEDLINE |
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